3D Nanofiber-Assisted Embedded Extrusion Bioprinting for Oriented Cardiac Tissue Fabrication

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-10-19 DOI:10.1021/acsbiomaterials.4c0161110.1021/acsbiomaterials.4c01611

Huiquan Wu, Feng Xu, Hang Jin, Mingcheng Xue, Wangzihan Zhang, Jianhui Yang, Junyi Huang, Yuqing Jiang, Bin Qiu, Bin Lin, Qiang Gao, Songyue Chen* and Daoheng Sun*,

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Abstract

Three-dimensional (3D) bioprinting technology stands out as a promising tissue manufacturing process to control the geometry precisely with cell-loaded bioinks. However, the isotropic culture environment within the bioink and the lack of topographical cues impede the formation of oriented cardiac tissue. To overcome this limitation, we present a novel method named 3D nanofiber-assisted embedded bioprinting (3D-NFEP) to fabricate cardiac tissue with an oriented morphology. Aligned 3D nanofiber scaffolds were fabricated by divergence electrospinning, which provided structural support for printing of the low-viscosity bioink and structural induction to cardiomyocytes. Cells adhered to the aligned fibers after hydrogel degradation, and a high degree of cell alignment was observed. This technology was also demonstrated as a feasible solution for multilayer cell printing. Therefore, 3D-NFEP was demonstrated as a promising method for bioprinting oriented cardiac tissue with low-viscosity bioink and is expected to be applied for structured and cardiac tissue engineering.

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用于定向心脏组织制造的三维纳米纤维辅助嵌入式挤压生物打印技术

三维（3D）生物打印技术是一种前景广阔的组织制造工艺，它能利用装载细胞的生物墨水精确控制几何形状。然而，生物墨水内各向同性的培养环境和地形线索的缺乏阻碍了定向心脏组织的形成。为了克服这一限制，我们提出了一种名为三维纳米纤维辅助嵌入式生物打印（3D-NFEP）的新方法，用于制造具有定向形态的心脏组织。通过发散电纺丝制造出排列整齐的三维纳米纤维支架，为低粘度生物墨水的打印和心肌细胞的结构诱导提供了结构支持。细胞在水凝胶降解后附着在排列整齐的纤维上，并观察到细胞高度排列整齐。这项技术还被证明是多层细胞打印的可行解决方案。因此，3D-NFEP 被证明是用低粘度生物墨水进行定向心脏组织生物打印的一种有前途的方法，有望应用于结构和心脏组织工程。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture