Spray paint-derived microplastics and incorporated substances as ecotoxicological contaminants in the neotropical bumblebee Bombus atratus

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2024-11-06 DOI:10.1016/j.etap.2024.104586

Guilherme Andrade Neto Schmitz Boeing , Michele Provase , Elisabete Tsukada , Raquel F. Salla , Walter Ruggeri Waldman , Fábio Camargo Abdalla

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引用次数: 0

Abstract

While bumblebees may be exposed to microplastics (MPs), the effects on them are not well studied. Therefore, in this research, we assessed the cytotoxicity of pristine and photodegraded spray paint-derived MPs on the midgut, Malpighian tubules, and hepato-nephrocitic system cells of Bombus atratus workers exposed to 50 mg.L⁻¹ MPs for 96 hours. Histological and histochemical analyses revealed that pristine MPs caused subtle cellular changes, while the exposure to photodegraded MPs led to significant vacuolization, nuclear condensation, and pyknosis. These effects are possibly linked to the release of potentially toxic elements (PTEs) like Copper, Manganese, and Iron from photodegraded MPs, which exceeded Brazil's CONAMA safety limits. Photodegraded MPs also reduced body weight, disrupting homeostasis and potentially decreasing bumblebee’s fitness. These findings highlight the importance of studying the toxicity of environmentally realistic MPs, as plastic composition and weathering significantly influence their harmful effects.

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喷漆衍生的微塑料和掺杂物质是新热带熊蜂Bombus atratus体内的生态毒理学污染物。

虽然大黄蜂可能会接触到微塑料（MPs），但对其影响的研究并不多。因此，在这项研究中，我们评估了原始和光降解喷漆衍生的 MPs 对暴露于 50 mg.L-1 MPs 96 小时的大黄蜂工人的中肠、马氏管和肝肾系统细胞的细胞毒性。组织学和组织化学分析显示，原始 MPs 会导致细胞发生细微变化，而接触光降解 MPs 则会导致细胞明显空泡化、核凝结和细胞凋亡。这些影响可能与光降解 MPs 释放的潜在有毒元素（PTEs）有关，如铜、锰和铁，这些元素超过了巴西 CONAMA 的安全限制。光降解 MPs 还降低了体重，破坏了体内平衡，可能会降低大黄蜂的体能。这些发现凸显了研究现实环境中 MPs 的毒性的重要性，因为塑料成分和风化作用会对其有害影响产生重大影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.