Quercetin promotes the recovery of iron chlorosis in strawberry plants

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-03 DOI:10.1016/j.plaphy.2024.109266

Maribela Pestana , Teresa Saavedra , Florinda Gama , Maria A. Rodrigues , Amarilis de Varennes , José Paulo Da Silva , Pedro José Correia

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引用次数: 0

Abstract

Iron (Fe) chlorosis is very common in plants cultivated in calcareous soils of the Mediterranean basin and is usually corrected by the application of Fe chelates to the soil, which can have a negative impact on the environment. The aim of this experiment was to assess the role of quercetin, a natural compound widely present in plants and known to bind Fe, in correcting Fe chlorosis when supplied in the Hoagland nutrient solution. In this context, strawberry plants were grown at different Fe concentrations, with 0 (Fe0), 1 (Fe1) and 5 (Fe5) μM of Fe in the nutrient solution, until the onset of clear Fe chlorosis symptoms. Subsequently, the recovery of Fe chlorosis was investigated through the addition of Fe and/or quercetin (Q) to nutrient solutions. Throughout the experiment, leaf chlorophyll (Chl) was estimated using the SPAD values. The root ferric chelate-reductase (FCR) activity was determined in the root apices, and the foliar Fe concentration was also quantified. At the end of the experiment, plants grown without Fe but supplemented with Fe1 plus quercetin [Fe0+(Fe1+Q)] recovered completely from Fe chlorosis and showed a Chl concentration (700–800 μmol m⁻²) in young leaves similar to that observed in control plants (Fe5). The remaining treatments exhibited lower Chl concentrations, with values ranging from 92.4 to 217.0 μmol of Chl per m². FCR activity was approximately five-to six-fold higher in the Fe0+(Fe1+Q) treatment than in the Fe0 and Fe5 treatments. However, the plants that were consistently grown with Fe in the nutrient solution (Fe1 and Fe5) exhibited the highest Fe content in their leaves. The findings suggest that quercetin has the potential to function as an Fe complexing agent, thereby enhancing the recovery of strawberry plants with Fe deficiency.

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槲皮素能促进草莓植株铁萎黄病的恢复。

在地中海盆地的石灰性土壤中栽培植物，铁（Fe）萎绿症非常常见，通常通过在土壤中施用铁螯合物来纠正，但这可能会对环境造成负面影响。本实验的目的是评估槲皮素（一种广泛存在于植物中的天然化合物，已知可与铁结合）在胡格兰营养液中对纠正铁萎黄病的作用。在这种情况下，草莓植株在不同的铁浓度下生长，营养液中的铁含量分别为 0（Fe0）、1（Fe1）和 5（Fe5）微摩尔，直到出现明显的铁褪绿症状。随后，通过在营养液中添加铁和/或槲皮素（Q），研究了铁萎黄病的恢复情况。在整个实验过程中，叶片叶绿素（Chl）是通过 SPAD 值估算的。根尖的铁螯合还原酶（FCR）活性被测定，叶片铁浓度也被量化。实验结束时，未添加铁元素但添加了 Fe1 和槲皮素的植株[Fe0+(Fe1+Q)]完全摆脱了铁元素萎黄病，其嫩叶中的 Chl 浓度（700-800 μmol m-2）与对照植株（Fe5）中的浓度相似。其余处理的叶绿素浓度较低，从每平方米 92.4 到 217.0 μmol 不等。与 Fe0 和 Fe5 处理相比，Fe0+（Fe1+Q）处理的 FCR 活性大约高出 5 到 6 倍。然而，在营养液中持续添加铁元素的植株（Fe1 和 Fe5）叶片中的铁元素含量最高。研究结果表明，槲皮素有可能作为一种铁络合剂，从而提高缺铁草莓植株的恢复能力。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.