Discovery and functional characterization of two novel glycosyltransferases associated with the biosynthesis of α-hederin in Dipsacus asperoides.

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-05 DOI:10.1016/j.plaphy.2024.109273

Weilin Yao, Tengfei Niu, Jie Pan, Xiaolin Yang, Chaokang Huang, Huida Guan, Li Yang, Zhengtao Wang, Rufeng Wang

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引用次数: 0

Abstract

Triterpenoid saponins are crucial natural products widely distributed in various medicinal plants, with Dipsacus asperoides being particularly rich in these compounds. However, the glycosyltransferases responsible for the biosynthesis of α-hederin, one of the primary bioactive secondary metabolites in D. asperoides, have not been elucidated. In this study, transcriptomic and compound analyses revealed 359 differentially expressed genes associated with secondary metabolism, with 271 involved in triterpenoid saponin glycosylation. Through correlation analysis, 71 candidate glycosyltransferases were identified, and two novel glycosyltransferases were functionally characterized. It was shown that DaUGT121 catalyzes the conversion of hederagenin into cauloside A, while DaUGT103 acts as a cauloside A 1,2-rhamnosyltransferase transforming cauloside A into α-hederin. These findings illuminate the biosynthesis of triterpenoid saponins in D. asperoides, providing insights into the molecular mechanisms and offering novel tools for synthesizing natural products with diverse sugar moieties.

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发现与 Dipsacus asperoides 中 α-hederin 生物合成有关的两种新型糖基转移酶并确定其功能特征。

三萜类皂苷是广泛分布于各种药用植物中的重要天然产物，其中 Dipsacus asperoides 尤为富含此类化合物。然而，负责α-hederin（D. asperoides 的主要生物活性二级代谢产物之一）生物合成的糖基转移酶尚未阐明。在这项研究中，转录组和化合物分析发现了 359 个与次生代谢相关的差异表达基因，其中 271 个基因参与了三萜类皂苷的糖基化。通过相关分析，确定了 71 个候选糖基转移酶，并对两个新型糖基转移酶进行了功能鉴定。研究表明，DaUGT121 可催化赫尔德皂苷向 cauloside A 的转化，而 DaUGT103 则作为 cauloside A 1,2 鼠李糖基转移酶，将 cauloside A 转化为 α-hederin。这些发现揭示了 D. asperoides 三萜类皂苷的生物合成过程，提供了对分子机制的深入了解，并为合成具有不同糖分子的天然产品提供了新的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.