Feline cumulus cells and oocytes show massive accumulation of lipid droplets and upregulation of PLIN2 expression after in vitro maturation

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2024-11-01 DOI:10.1016/j.theriogenology.2024.10.031

Natalia Sowinska , Joanna Lechtanska , Katarzyna Greczka , Dorota Lechniak , Piotr Pawlak

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引用次数: 0

Abstract

This study investigates lipid content and expression of genes involved in lipid metabolism in feline cumulus cells and oocytes before and after in vitro maturation (IVM). Domestic cats represent valuable models in reproductive research, yet the efficiency of in vitro embryo production remains suboptimal, with contributing factors still under investigation. We characterized lipid droplets (LDs) in oocytes collected from adult queens, both before and after IVM, using confocal microscopy with Bodipy 493/503 staining. We also quantified the expression of four genes involved in regulation of lipid metabolism. Our findings revealed a substantial accumulation of LDs and a significant upregulation of PLIN2 expression in both cumulus cells and oocytes following IVM. The number and total area of LDs and fluorescence intensity increased markedly in oocytes at the MII stage, while the average LD diameter decreased. Similarly, cumulus cells showed an increase in number and total area of LDs post-IVM, suggesting their involvement in oocyte maturation by modulating lipid homeostasis. This study presents the first detailed characterization of lipid droplet dynamics in feline oocytes and cumulus cells, providing insights into metabolic processes during IVM, which are critical for optimizing assisted reproductive technologies in felids.

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猫精原细胞和卵母细胞在体外成熟后会出现大量脂滴堆积和 PLIN2 表达上调。

本研究调查了体外成熟（IVM）前后猫积液细胞和卵母细胞中的脂质含量和脂质代谢相关基因的表达。家猫是生殖研究的宝贵模型，但体外胚胎生产的效率仍不理想，其原因仍在调查之中。我们利用共聚焦显微镜和 Bodipy 493/503 染色技术对从成年雌猫卵母细胞中收集的脂滴（LDs）进行了表征，包括 IVM 之前和之后。我们还量化了参与调控脂质代谢的四个基因的表达。我们的研究结果表明，IVM 后，积层细胞和卵母细胞中的 LDs 大量积累，PLIN2 的表达显著上调。在 MII 阶段的卵母细胞中，LDs 的数量和总面积以及荧光强度明显增加，而 LD 的平均直径则有所下降。同样，积层细胞在 IVM 后显示出 LDs 数量和总面积的增加，这表明积层细胞通过调节脂质平衡参与了卵母细胞的成熟。这项研究首次详细描述了猫科动物卵母细胞和积层细胞中脂滴的动态特征，为了解体外受精过程中的新陈代谢过程提供了见解，这对于优化猫科动物的辅助生殖技术至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.