Mitchel C Schiewe, Ryan Reichelderfer, Kathryn Wozniak, Claudia De Romana, Melanie Nordbak, Kelly Baek, Karine Chung
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引用次数: 0
Abstract
Research question: Can GV-oocytes serve as an effective model to test the efficacy of ultra-fast vitrification (UFV)/ rapid elution (RE) treatments to support reliable, high survival rates and sustained functionality?
Design: Prospective pilot cohort studies were performed to investigate the feasibility of non-equilibration, UFV to sustain cellular integrity and development in contrast to control vitrification (CV: 10-15min ES/ 1min VS). In Phase 1, we applied a 2 × 2 factorial design (n=25-30 eggs/group) to evaluate post-warming dilution treatments: conventional multi-step (CD) versus rapid elution (RE; one-step), including an apriori fresh egg control group. Phase 1/2 focused on survival and maturation assessments, including meiotic spindle formation (Phase 2).
Results: The survival of EG/DMSO treated UFV oocytes in Phase 1 and 2 was not different to spontaneous degeneration seen in the fresh IVM control groups (3.2%) but was higher than CV treated oocytes immediately post-warming (p<0.03). Of the intact GVs, no difference in IVM-MII development was detected (52.6 -58.3%) at +48h IVM across all groups. Meiotic spindle integrity of MII oocytes was normal in all treatment groups.
Conclusions: As originally reported by Gallardo (2019), non-equilibrated dehydrated human oocytes can effectively vitrify after UFV/CD treatment. We further verified the resiliency of oocytes to withstand RE treatment and continue to develop normally, like fresh GV-matured oocytes. Furthermore, we confirmed that the meiotic spindle formation and density of UFV/RE-treated GV oocytes was similar to fresh controls. Overall, the GV-model proved to be a useful resource to substantiate the promising potential of UFV technology to reliably achieve high survival and normal developmental competence in a more time efficient manner.
期刊介绍：
Reproductive BioMedicine Online covers the formation, growth and differentiation of the human embryo. It is intended to bring to public attention new research on biological and clinical research on human reproduction and the human embryo including relevant studies on animals. It is published by a group of scientists and clinicians working in these fields of study. Its audience comprises researchers, clinicians, practitioners, academics and patients.
Context:
The period of human embryonic growth covered is between the formation of the primordial germ cells in the fetus until mid-pregnancy. High quality research on lower animals is included if it helps to clarify the human situation. Studies progressing to birth and later are published if they have a direct bearing on events in the earlier stages of pregnancy.
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