A 4D time-lapse morphometry method to quantify bone formation and resorption during distraction osteogenesis.

IF 2.1 3区 医学 Q2 ORTHOPEDICS
Sishun Pu, Ruisen Fu, David Bertrand, Bettina M Willie, Haisheng Yang
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引用次数: 0

Abstract

Distraction osteogenesis (DO) is widely utilized for treating limb length discrepancy, nonunion, bone deformities and defects. This study sought to develop a 4D time-lapse morphometry method to quantify bone formation and resorption in mouse femur during DO based on image registration of longitudinal in vivo micro-CT scans. Female C57BL/6 mice (n = 7) underwent osteotomy, followed by 5 days of latency, 10 days of distraction and 35 days of consolidation. The mice were scanned with micro-CT at Days 5, 15, 25, 35, 45, and 50. Histological sectioning and Movat Pentachrome straining were performed at Day 50. After registration of two consecutive micro-CT images of the same bone (day x and day y), the spatially- and temporally-linked sequences of formation, resorption and quiescent bones at the distraction gap were identified and bone formation and resorption rates (BFRdayx-y and BRRdayx-y) were calculated. The overall percentage error of the registration method was 2.98% ± 0.89% and there was a strong correlation between histologically-measured bone area fraction and micro-CT-determined bone volume fraction at Day 50 (r = 0.89, p < 0.05). The 4D time-lapse morphometry indicated a rapid bone formation during the first 10 days of the consolidation phase (BFRday15-25 = 0.14 ± 0.05 mm3/day), followed by callus reshaping via equivalent bone formation and resorption rates. The 4D time-lapse morphometry method developed in this study allows for a continuous quantitative monitoring of the dynamic process of bone formation and resorption following distraction, which may offer a better understanding of the mechanism for mechano-regulated bone regeneration and aid for development of new treatment strategies of DO.

在牵引成骨过程中量化骨形成和吸收的四维延时形态测量法。
牵引成骨(DO)被广泛用于治疗肢体长度不一致、骨不连、骨畸形和缺损。本研究试图开发一种四维延时形态测量法,根据纵向活体显微CT扫描的图像配准,量化小鼠股骨在牵引成骨过程中的骨形成和骨吸收。雌性 C57BL/6 小鼠(n = 7)接受截骨术,然后是 5 天的潜伏期、10 天的牵引期和 35 天的巩固期。分别在第 5、15、25、35、45 和 50 天对小鼠进行 micro-CT 扫描。在第 50 天进行组织切片和 Movat Pentachrome 染色。对同一骨骼的两张连续 micro-CT 图像(第 x 天和第 y 天)进行配准后,确定牵引间隙处骨骼形成、吸收和静止的空间和时间关联序列,并计算骨骼形成率和吸收率(BFRdayx-y 和 BRRdayx-y)。登记方法的总体百分比误差为 2.98% ± 0.89%,在第 50 天时,组织学测量的骨面积分数与 micro-CT 确定的骨体积分数之间有很强的相关性(r = 0.89,p day15-25 = 0.14 ± 0.05 mm3/天),随后通过等效的骨形成和吸收率进行胼胝体重塑。本研究开发的四维延时形态测量法可对牵引后骨形成和吸收的动态过程进行连续定量监测,从而更好地了解机械调控骨再生的机制,并有助于开发新的 DO 治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Orthopaedic Research®
Journal of Orthopaedic Research® 医学-整形外科
CiteScore
6.10
自引率
3.60%
发文量
261
审稿时长
3-6 weeks
期刊介绍: The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.
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