Whole-Genome Sequencing And Characterization Of Two Bacillus velezensis Strains from Termitarium and A Comprehensive Comparative Genomic Analysis of Biosynthetic Gene Clusters.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Venkatesan Dhanalakshmi, Jeyaprakash Rajendhran
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Abstract

The species Bacillus velezensis is known for its biosynthetic potential and metabolic versatility in producing several secondary metabolites and promoting plant growth. In this study, we isolated two B. velezensis strains, WGTg-8 and WGTm-299, from the termite gut and termitarium, which exhibited antimicrobial activity against multiple clinical and phytopathogens. The whole genomes of these strains were sequenced using the Illumina platform and annotated. The genome mining of the draft genome sequences revealed 48 biological gene clusters (BGCs) responsible for synthesizing various secondary metabolites. The construction of the similarity network of the BGCs and the comparative analysis with the genetically related organisms are aided in the identification of metabolites produced by these strains. We identified biosynthetic gene clusters (BGCs) coding for macrolactin H, bacilysin, bacillibactin, amylocyclin, comX4, and LCI, found in both strains with 100% similarity. The difficidin, bacillaene, thusin_alpha, and cericidin BGCs are exclusively found in strain WGTg-8, while the colicin BGC is exclusively present in the WGTm-299 strain. The fengycin and surfactin gene clusters are present in both strains with 80% similarity. Furthermore, 28 putative NRPS BGCs, NRPS-T1PKS hybrid clusters, a T1PKS, and a bacteriocin BGC were identified with very low similarity (≤ 25%) or no similarity with known antibiotics. In addition, we found several genes coding for plant growth-promoting properties, including nitrogen metabolism, hormone synthesis, sulfur metabolism, phosphate metabolism, and a few other properties.

来自白蚁的两株 Velezensis 杆菌的全基因组测序和特性分析以及生物合成基因簇的综合比较基因组分析
枯草芽孢杆菌(Bacillus velezensis)以其生物合成潜力和代谢多功能性而闻名,可产生多种次级代谢产物并促进植物生长。在本研究中,我们从白蚁肠道和白蚁房中分离出两株 B. velezensis 菌株 WGTg-8 和 WGTm-299,它们对多种临床病原体和植物病原体具有抗菌活性。利用 Illumina 平台对这些菌株的全基因组进行了测序和注释。对基因组序列草案的基因组挖掘发现了 48 个负责合成各种次生代谢物的生物基因簇(BGCs)。这些生物基因组的相似性网络的构建以及与遗传相关生物的比较分析有助于鉴定这些菌株产生的代谢物。我们确定了两个菌株中的生物合成基因簇(BGCs),它们分别编码大乳酸素 H、杆菌素、杆菌肽、淀粉环素、comX4 和 LCI,相似度达到 100%。艰难素、杆菌素、茄素_α和cericidin BGC只存在于WGTg-8菌株中,而大肠杆菌素BGC只存在于WGTm-299菌株中。两种菌株中都存在芬吉素和表面活性素基因簇,相似度高达 80%。此外,我们还发现了 28 个假定的 NRPS BGC、NRPS-T1PKS 杂交基因簇、一个 T1PKS 和一个细菌素 BGC,它们与已知抗生素的相似度非常低(≤ 25%)或没有相似度。此外,我们还发现了几个编码促进植物生长特性的基因,包括氮代谢、激素合成、硫代谢、磷代谢和其他一些特性。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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