Evaluation of usage of readily accessible Enterobacteriaceae differential and selective media for identifying Candida auris

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-30 DOI:10.1016/j.diagmicrobio.2024.116589

Hossein Khodadadi , Esmaeil Eghtedarnejad , Amirhossein Ahmadi , Ali Khodadadi , Neda Shamsdin

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引用次数: 0

Abstract

Background

Candida auris is a drug-resistant fungal pathogen. Diagnosing it is challenging due to the need for modern techniques. This study aims to evaluate the usefulness of Enterobacteriaceae culture media in screening and identifying Candida auris, as those media are accessible in resource-limited laboratories.

Method

The study used various yeast strains, including Candida auris, to challenge bacteriologic media and evaluated the effectiveness of different Enterobacteriaceae differential and selective media in identifying and differentiating Candida auris from other yeasts.

Results

All yeasts can grow on all Enterobacteriaceae differential and selective media during various incubation times, resulting in variable colony sizes. Simon's Citrate Agar medium can differentiate Candida auris and other members of C. haemulonii complex from other yeasts.

Conclusion

Although definitive identification of Candida auris is challenging and requires specific methods, Citrate Agar could be a preliminary screening method in source-limited regions.

查看原文本刊更多论文

评估使用易于获取的肠杆菌科鉴别培养基和选择性培养基鉴定白色念珠菌的情况。

背景：白色念珠菌是一种耐药性真菌病原体。由于需要现代技术，对其进行诊断具有挑战性。本研究旨在评估肠杆菌科培养基在筛选和鉴定念珠菌方面的作用，因为资源有限的实验室可以使用这些培养基：方法：本研究使用包括白色念珠菌在内的各种酵母菌株挑战细菌培养基，并评估不同的肠杆菌科鉴别培养基和选择性培养基在鉴定和区分白色念珠菌与其他酵母菌方面的有效性：结果：在不同的培养时间内，所有酵母菌都能在所有的肠杆菌科鉴别培养基和选择性培养基上生长，从而产生不同大小的菌落。西蒙柠檬酸盐琼脂培养基可将白色念珠菌和血色念珠菌复合体的其他成员与其他酵母菌区分开来：结论：尽管明确鉴定白色念珠菌具有挑战性，需要特定的方法，但柠檬酸盐琼脂可以作为来源有限地区的初步筛选方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.