Dingkun Zhang, Xin Li, Wen Zheng, Luolan Gui, Wenjuan Zeng, Yu Zeng, Yin Yang, Rong Fan, Yang Lu, Yueqiu Liu, Xinyi Hu, Ning Mao, Junwen Guan, Tao Li, Jingqiu Cheng, Hao Yang, Meng Gong
{"title":"Probing the biocompatibility of Mo2C nanosheet through an integrated metabolomics approach: Toward boosting energy metabolism.","authors":"Dingkun Zhang, Xin Li, Wen Zheng, Luolan Gui, Wenjuan Zeng, Yu Zeng, Yin Yang, Rong Fan, Yang Lu, Yueqiu Liu, Xinyi Hu, Ning Mao, Junwen Guan, Tao Li, Jingqiu Cheng, Hao Yang, Meng Gong","doi":"10.1116/6.0003872","DOIUrl":null,"url":null,"abstract":"<p><p>An Mo2C nanosheet is an important two-dimensional nanomaterial with distinguished catalytic activity in biochemical applications. However, detailed information on Mo2C-induced changes in metabolic shifts, biosafety, and molecular mechanisms is insufficient. Integrated metabolomics (including aqueous metabolomics, lipidomics, and spatial metabolomics) has provided an excellent choice with massive bioinformation. In addition, the notion of \"nanometabolomics\" was first proposed and utilized to refer to these metabolomics studies on the biosafety, biocompatibility, and biological response of nanomaterials. Nanometabolomics innovatively combined nanoscience and metabolomics with massive bioinformation at the molecular level. For instance, in this work, nanometabolomics specialized in probing an Mo2C-induced metabolic shift of human umbilical vein endothelial cells (HUVECs) through integrated metabolomics. Furthermore, integrated metabolomics was used to examine the metabolic shift of HUVECs at the metabolome and lipidome levels, as well as the spatial distribution of different metabolites. The findings demonstrated that high doses (1 mg/ml) of an Mo2C nanosheet might produce an immediate improvement in HUVECs' energy metabolism, which was closely related to the improved morphology and function of mitochondria. The integrated metabolomics outcomes of this unique \"Mo2C-cell\" system increased our understanding of an Mo2C nanosheet. The proposed new word \"nanometabolomics\" could also be considered an excellent notion in representing nanomaterial-involved metabolomics studies.</p>","PeriodicalId":9053,"journal":{"name":"Biointerphases","volume":"19 6","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biointerphases","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1116/6.0003872","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
An Mo2C nanosheet is an important two-dimensional nanomaterial with distinguished catalytic activity in biochemical applications. However, detailed information on Mo2C-induced changes in metabolic shifts, biosafety, and molecular mechanisms is insufficient. Integrated metabolomics (including aqueous metabolomics, lipidomics, and spatial metabolomics) has provided an excellent choice with massive bioinformation. In addition, the notion of "nanometabolomics" was first proposed and utilized to refer to these metabolomics studies on the biosafety, biocompatibility, and biological response of nanomaterials. Nanometabolomics innovatively combined nanoscience and metabolomics with massive bioinformation at the molecular level. For instance, in this work, nanometabolomics specialized in probing an Mo2C-induced metabolic shift of human umbilical vein endothelial cells (HUVECs) through integrated metabolomics. Furthermore, integrated metabolomics was used to examine the metabolic shift of HUVECs at the metabolome and lipidome levels, as well as the spatial distribution of different metabolites. The findings demonstrated that high doses (1 mg/ml) of an Mo2C nanosheet might produce an immediate improvement in HUVECs' energy metabolism, which was closely related to the improved morphology and function of mitochondria. The integrated metabolomics outcomes of this unique "Mo2C-cell" system increased our understanding of an Mo2C nanosheet. The proposed new word "nanometabolomics" could also be considered an excellent notion in representing nanomaterial-involved metabolomics studies.
期刊介绍:
Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee.
Topics include:
bio-surface modification
nano-bio interface
protein-surface interactions
cell-surface interactions
in vivo and in vitro systems
biofilms / biofouling
biosensors / biodiagnostics
bio on a chip
coatings
interface spectroscopy
biotribology / biorheology
molecular recognition
ambient diagnostic methods
interface modelling
adhesion phenomena.