Chuanhui Du, Wenjie Sun, Qingwei Song, Kaijing Zuo
{"title":"GhDOFD45 promotes sucrose accumulation in cotton seeds by transcriptionally activating GhSWEET10 expression","authors":"Chuanhui Du, Wenjie Sun, Qingwei Song, Kaijing Zuo","doi":"10.1111/tpj.17123","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Cotton seed development and fiber elongation are the inseparable and overlapped development processes requiring the continuous supply of sucrose as the direct carbon source. However, little is known about the molecular mechanism of how sucrose is transported from the source tissues (leaves) into growing cotton seeds. Here, we identify the function of a sucrose transporter gene, <i>Sugars Will Eventually be Exported Transporter 10</i>, <i>GhSWEET10</i> in cotton seed development. <i>GhSWEET10</i> encodes a functional sucrose transporter, predominantly expressing in the funiculus, inner seedcoat, and endosperm during fiber elongation. <i>GhSWEET10</i> RNAi plants (<i>GhSWEET10i</i>) accumulated less sucrose and glucose in growing seeds and that led to shorter fibers and smaller seeds, whereas <i>GhSWEET10</i> overexpressed plants (<i>GhSWEET10OE</i>) had bigger seeds and longer fibers with more sugar accumulation during fiber elongation. <i>GhSWEET10</i> gene is transcriptionally controlled by the transcription factor GhDOFD45. <i>GhDOFD45</i> knockout plants (<i>GhDOFD45-KO</i>) possessed the phenotypes of smaller seeds and shorter fibers like those of <i>GhSWEET10i</i> plants. Furthermore, GhSWEET10 mainly exports the sucrose from the funiculus into developing seeds according to the mimic-analysis of sucrose transporting. Collectively, all these findings show that GhDOFD45 positively regulates <i>GhSWEET10</i> expression to mainly transport sucrose from leaves into developing cotton seeds. Our findings also imply that the sucrose transport into enlarging seeds benefits fiber development, and thus <i>GhSWEET10</i> can be selected as a target of breeding novel cotton varieties with larger and more vigorous seeds.</p>\n </div>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2468-2484"},"PeriodicalIF":6.2000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17123","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Cotton seed development and fiber elongation are the inseparable and overlapped development processes requiring the continuous supply of sucrose as the direct carbon source. However, little is known about the molecular mechanism of how sucrose is transported from the source tissues (leaves) into growing cotton seeds. Here, we identify the function of a sucrose transporter gene, Sugars Will Eventually be Exported Transporter 10, GhSWEET10 in cotton seed development. GhSWEET10 encodes a functional sucrose transporter, predominantly expressing in the funiculus, inner seedcoat, and endosperm during fiber elongation. GhSWEET10 RNAi plants (GhSWEET10i) accumulated less sucrose and glucose in growing seeds and that led to shorter fibers and smaller seeds, whereas GhSWEET10 overexpressed plants (GhSWEET10OE) had bigger seeds and longer fibers with more sugar accumulation during fiber elongation. GhSWEET10 gene is transcriptionally controlled by the transcription factor GhDOFD45. GhDOFD45 knockout plants (GhDOFD45-KO) possessed the phenotypes of smaller seeds and shorter fibers like those of GhSWEET10i plants. Furthermore, GhSWEET10 mainly exports the sucrose from the funiculus into developing seeds according to the mimic-analysis of sucrose transporting. Collectively, all these findings show that GhDOFD45 positively regulates GhSWEET10 expression to mainly transport sucrose from leaves into developing cotton seeds. Our findings also imply that the sucrose transport into enlarging seeds benefits fiber development, and thus GhSWEET10 can be selected as a target of breeding novel cotton varieties with larger and more vigorous seeds.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.