Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection

IF 11.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2024-11-13 DOI:10.1126/sciadv.adq2899

Wenshang Guo, Ye Tao, Ruizhe Yang, Kaihao Mao, Hongwei Zhou, Minghui Xu, Tie Sun, Xiao Li, Changrui Shi, Zhenyou Ge, Rui Xue, Haizhou Zhou, Yukun Ren

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引用次数: 0

Abstract

Developing instant detection systems with disease diagnostic capabilities holds immense importance for remote or resource-limited areas. However, the task of creating these systems—which are simultaneously easy to operate, rapid in detection, and cost-effective—remains a challenge. In this study, we present a compact highly sensitive photothermal reverse transcriptase–loop-mediated isothermal amplification (RT-LAMP) chip (SPRC) designed for the detection of multiple diseases. The nucleic acid (NA) amplification on the chip is achieved through LAMP driven by either LED illumination or simple sunlight focusing. SPRC performs sample addition and amplification within a limited volume and autonomous enrichment of NA during the sample addition process, achieving a limit of detection (LOD) as low as 0.2 copies per microliter. Through 120 clinical samples, we achieved an accuracy of 95%, with a specificity exceeding 97.5%. Overall, SPRC has achieved promising progress in the application of point-of-care testing (POCT) by using light energy to simultaneously detect multiple diseases.

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用于同时检测多种疾病的紧凑型高灵敏度光热 RT-LAMP 芯片

开发具有疾病诊断能力的即时检测系统对于偏远地区或资源有限的地区具有重要意义。然而，如何开发出操作简便、检测快速、成本低廉的系统仍是一项挑战。在这项研究中，我们提出了一种紧凑型高灵敏度光热反转录酶环介导等温扩增（RT-LAMP）芯片（SPRC），设计用于检测多种疾病。芯片上的核酸（NA）扩增是通过 LED 照明或简单的阳光聚焦驱动的 LAMP 实现的。SPRC 在有限的体积内进行样品添加和扩增，并在样品添加过程中自主富集 NA，检测限（LOD）低至每微升 0.2 个拷贝。通过 120 份临床样本，我们的准确率达到 95%，特异性超过 97.5%。总之，SPRC 利用光能同时检测多种疾病，在应用床旁检测（POCT）方面取得了可喜的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.