Production and application of inulinase by new isolates of Aspergillus welwitschiae from fermented peach-palm waste for the production of fructooligosaccharides

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-12 DOI:10.1016/j.foodchem.2024.141978

Emanuelle Santos de Carvalho Cardoso, Nívea Queiroz Martins, Raquel Araújo Azevedo, Lucas Sousa Palmeira, Gabriel Quintanilha-Peixoto, Bruno Andrade, Mateus Pereira Flores Santos, Ana Paula Trovatti Uetanabaro, Erik Galvão Paranhos da Silva, Aristóteles Góes-Neto, Andréa Miura da Costa

{"title":"Production and application of inulinase by new isolates of Aspergillus welwitschiae from fermented peach-palm waste for the production of fructooligosaccharides","authors":"Emanuelle Santos de Carvalho Cardoso, Nívea Queiroz Martins, Raquel Araújo Azevedo, Lucas Sousa Palmeira, Gabriel Quintanilha-Peixoto, Bruno Andrade, Mateus Pereira Flores Santos, Ana Paula Trovatti Uetanabaro, Erik Galvão Paranhos da Silva, Aristóteles Góes-Neto, Andréa Miura da Costa","doi":"10.1016/j.foodchem.2024.141978","DOIUrl":null,"url":null,"abstract":"Microbial inulinase enzymes have a number of applications in biotechnology. In this study, new strains of Aspergillus welwitschiae were investigated as producers of inulinases and their endo- and exo-inulases were characterized in silico and their protein modeling was performed. The inulinase production by A. welwitschiae employing the Dohelert method to assess the interaction between temperature and cultivation humidity, resulted in a fourfold increase in activity. The optimal temperature and humidity were 25–27 °C and 75–82 %, respectively. The enzyme exhibited optimal activity at a pH of 3.5 and at 60 °C. The hydrolysis of the sisal root yielded 272 mg/g of reducing sugars and oligosaccharides. Our in silico experiments predicted 10 well-validated structures of endo- (5) and exo-inulinases (5) from A. welwitschiae. The notable activity of these inulinases on inulin highlights their potential for the treatment of agro-industrial residues with the objective of producing high-value added products.","PeriodicalId":8,"journal":{"name":"ACS Biomaterials Science & Engineering","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Biomaterials Science & Engineering","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2024.141978","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Microbial inulinase enzymes have a number of applications in biotechnology. In this study, new strains of Aspergillus welwitschiae were investigated as producers of inulinases and their endo- and exo-inulases were characterized in silico and their protein modeling was performed. The inulinase production by A. welwitschiae employing the Dohelert method to assess the interaction between temperature and cultivation humidity, resulted in a fourfold increase in activity. The optimal temperature and humidity were 25–27 °C and 75–82 %, respectively. The enzyme exhibited optimal activity at a pH of 3.5 and at 60 °C. The hydrolysis of the sisal root yielded 272 mg/g of reducing sugars and oligosaccharides. Our in silico experiments predicted 10 well-validated structures of endo- (5) and exo-inulinases (5) from A. welwitschiae. The notable activity of these inulinases on inulin highlights their potential for the treatment of agro-industrial residues with the objective of producing high-value added products.

查看原文本刊更多论文

从发酵的桃棕榈废料中分离出的韦氏曲霉新菌株生产菊粉酶及其在果寡糖生产中的应用

微生物菊粉酶在生物技术领域有许多应用。本研究研究了作为菊粉酶生产者的韦氏曲霉（Aspergillus welwitschiae）的新菌株，并对其内在和外在菊粉酶进行了硅学表征和蛋白质建模。采用 Dohelert 法评估温度和培养湿度之间的相互作用，发现韦氏酵母菌生产的菊粉酶活性提高了四倍。最佳温度和湿度分别为 25-27 °C 和 75-82%。该酶在 pH 值为 3.5 和 60 °C 时表现出最佳活性。水解剑麻根可产生 272 毫克/克的还原糖和低聚糖。我们在硅学实验中预测了 10 个来自 A. welwitschiae 的内源性（5 个）和外源性（5 个）菊糖酶的有效结构。这些菊粉酶对菊粉的显著活性凸显了它们在处理农用工业残留物以生产高附加值产品方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

期刊介绍： ACS Biomaterials Science & Engineering is the leading journal in the field of biomaterials, serving as an international forum for publishing cutting-edge research and innovative ideas on a broad range of topics: Applications and Health – implantable tissues and devices, prosthesis, health risks, toxicology Bio-interactions and Bio-compatibility – material-biology interactions, chemical/morphological/structural communication, mechanobiology, signaling and biological responses, immuno-engineering, calcification, coatings, corrosion and degradation of biomaterials and devices, biophysical regulation of cell functions Characterization, Synthesis, and Modification – new biomaterials, bioinspired and biomimetic approaches to biomaterials, exploiting structural hierarchy and architectural control, combinatorial strategies for biomaterials discovery, genetic biomaterials design, synthetic biology, new composite systems, bionics, polymer synthesis Controlled Release and Delivery Systems – biomaterial-based drug and gene delivery, bio-responsive delivery of regulatory molecules, pharmaceutical engineering Healthcare Advances – clinical translation, regulatory issues, patient safety, emerging trends Imaging and Diagnostics – imaging agents and probes, theranostics, biosensors, monitoring Manufacturing and Technology – 3D printing, inks, organ-on-a-chip, bioreactor/perfusion systems, microdevices, BioMEMS, optics and electronics interfaces with biomaterials, systems integration Modeling and Informatics Tools – scaling methods to guide biomaterial design, predictive algorithms for structure-function, biomechanics, integrating bioinformatics with biomaterials discovery, metabolomics in the context of biomaterials Tissue Engineering and Regenerative Medicine – basic and applied studies, cell therapies, scaffolds, vascularization, bioartificial organs, transplantation and functionality, cellular agriculture