Glucosinolate profile and specifier protein activity determine the glucosinolate hydrolysis product formation in kohlrabi (Brassica oleracea var. gongylodes) in a tissue-specific way

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-13 DOI:10.1016/j.foodchem.2024.142032

Kudzai Gracious Mbudu, Katja Witzel, Frederik Börnke, Franziska Sabine Hanschen

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引用次数: 0

Abstract

Glucosinolates, commonly found in Brassica vegetables, are hydrolyzed by myrosinase to form bioactive isothiocyanates unless specifier proteins redirect the degradation to less bioactive nitriles and epithionitriles. Here, the tissue-specific impact of specifier proteins on the outcome of glucosinolate hydrolysis in nine kohlrabi tissues was investigated. Glucosinolates and their hydrolysis product profiles, epithiospecifier protein and myrosinase activity and protein abundance patterns of key glucosinolate biosynthesis, transport and hydrolysis proteins were determined and correlated to the metabolites in the kohlrabi tissues. Leaf tissues were rich in glucoraphanin, while bulb tissues contained more glucoerucin. Moreover, a higher proportion of isothiocyanates was formed in leaf stalk, bulb peel, stem and root, tissues with relatively higher epithiospecifier modifier 1 abundance and a higher ratio of myrosinase activity to ESP activity. This study shows that tissue-specific glucosinolate hydrolysis is mediated by glucosinolate biosynthesis and profiles, myrosinase activity and specifier protein as well as modifier protein abundance.

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葡萄糖苷酸谱和鉴定蛋白活性以组织特异性的方式决定苤蓝（Brassica oleracea var. gongylodes）中葡萄糖苷酸水解产物的形成

芸苔属蔬菜中常见的葡萄糖苷酸盐会被酪氨酸酶水解形成具有生物活性的异硫氰酸盐，除非指明蛋白将降解过程重新定向为生物活性较低的腈类和表硫腈类。在这里，我们研究了特异性蛋白质对九种苤蓝组织中葡萄糖苷酸水解结果的影响。研究人员测定了葡萄苷酸及其水解产物概况、表硫苷酸酶蛋白和酪氨酸酶活性以及关键葡萄苷酸生物合成、转运和水解蛋白的丰度模式，并将其与苤蓝组织中的代谢物进行了相关分析。叶片组织富含葡萄糖苷，而鳞茎组织则含有更多的葡萄糖苷。此外，在叶柄、鳞茎皮、茎和根等组织中，异硫氰酸酯的形成比例较高，这些组织的表硫修饰因子 1 丰度相对较高，酪氨酸酶活性与 ESP 活性的比例也较高。这项研究表明，组织特异性葡萄糖苷酸水解是由葡萄糖苷酸的生物合成和概况、酪氨酸酶活性和指明蛋白以及修饰蛋白丰度介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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