Conducting polymer functionalized Cu-metal organic framework–based electrochemical immunosensor for rapid and sensitive quantitation of Escherichia coli O157:H7

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-13 DOI:10.1007/s00604-024-06807-1

Xiaoge Duan, Xinrui Shi, Zhaoyuan He, Hongcai Chen, Zhuanzhuan Shi, Zhi Zhao, Hailan Chen, Meiling Yu, Chunxian Guo

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引用次数: 0

Abstract

Escherichia coli (E. coli) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of E. coli O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of E. coli O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (–SO₃H) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10² ~ 3 × 10⁸ cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting food safety and control of E. coli O157:H7 disease.

Graphical Abstract

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基于导电聚合物功能化铜金属有机框架的电化学免疫传感器，用于快速灵敏地定量检测大肠杆菌 O157:H7

大肠杆菌（E. coli）O157:H7 是一种重要的食源性致病菌，可导致人类和动物出血性腹泻和肠炎。实现大肠杆菌 O157:H7 的快速定量对保障食品安全和疾病控制具有重要意义。本研究开发了一种基于铜-金属有机框架（Cu-MOF）和聚（3,4-亚乙二氧基噻吩）-聚（苯乙烯磺酸）（PEDOT:PSS）功能化复合材料的电化学免疫传感技术，实现了对食品和临床粪便样品中大肠杆菌 O157:H7 的快速灵敏定量。Cu-MOF 的有机官能化大大提高了界面电导率，促进了电子转移，并提供了磺酸基团 (-SO3H) 来轭合生物识别元素以进行目标测定。该免疫传感器的线性检测范围为 3 × 102 ~ 3 × 108 cfu/mL，检测限（LOD）低至 7.4 cfu/mL，分析时间短至 40 分钟。此外，它与其他常见病原体没有交叉反应，重复性高，相对标准偏差（RSD）均低于 2.09%，为保证食品安全和控制大肠杆菌 O157:H7 疾病提供了一种可行的方法。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.