De novo luciferases enable multiplexed bioluminescence imaging

IF 9.6 1区化学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Materials Letters Pub Date : 2024-11-12 DOI:10.1016/j.chempr.2024.10.013

Julie Yi-Hsuan Chen, Qing Shi, Xue Peng, Jean de Dieu Habimana, James Wang, William Sobolewski, Andy Hsien-Wei Yeh

引用次数: 0

Abstract

We leverage AI-powered de novo protein design to create a new generation of luciferase catalysts, termed the neoLux series, which exhibit superior properties over native luciferases. These features include compact size, robust stability, cofactor independence, efficient cellular expression, higher catalytic efficiency, and unique substrate orthogonality, marking a significant advancement beyond the limitations of native luciferases. Additionally, we computationally designed highly efficient neoLux-fluorescent protein Förster resonance energy transfer (FRET) fusions capable of simultaneous multi-parametric imaging in cellulo and in vivo. Our pioneering approach has created a unified luminescent toolkit to allow for multi-colored tracking of cancer heterogeneity in vivo, paving the way for complex biological discovery.

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新荧光素酶实现了多重生物发光成像

我们利用人工智能驱动的从头蛋白质设计，创造出新一代荧光素酶催化剂（称为 neoLux 系列），与原生荧光素酶相比，它们具有更优越的特性。这些特性包括体积小巧、稳定性强、不依赖于辅助因子、细胞表达效率高、催化效率高以及独特的底物正交性，标志着超越原生荧光素酶限制的重大进步。此外，我们通过计算设计了高效的新荧光蛋白佛斯特共振能量转移（FRET）融合体，能够在细胞内和体内同时进行多参数成像。我们的开创性方法创建了一个统一的发光工具包，可在体内对癌症异质性进行多色追踪，为复杂的生物学发现铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Materials Letters MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.60

自引率

3.50%

发文量

261

期刊介绍： ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.