Integrated physiological, transcriptomic and metabolomic analyses of glossy mutant under drought stress in rapeseed (Brassica napus L.)

IF 5.6 1区 农林科学 Q1 AGRICULTURAL ENGINEERING
Ru Zhang , Ruolin Gong , Zhanling An, Guangze Li, Chunyan Dai, Rong Yi, Yaqian Liu, Jungang Dong, Jihong Hu
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引用次数: 0

Abstract

Rapeseed (Brassica napus L.), the second most important oil crop in the world, is extremely vulnerable to drought stress, resulting in severe reductions in yield and quality. Leaf epidermal wax plays an important role in drought stress. However, the genetic basis and regulatory mechanism of wax synthesis in B. napus remain unclear. Here, the integrated analysis of physiology, transcriptome, and metabolome was performed on a glossy mutant under drought stress to reveal the molecular basis of cuticular wax accumulation in rapeseed. Integrated transcriptomic and metabolomic analyses revealed the important roles of unsaturated fatty acids, wax biosynthesis, and plant hormone signal transduction in leaves for enhancing drought tolerance. Several candidate genes were identified to be involved in alkane synthesis and alcohol synthesis of the wax synthesis pathway, including CER1, MYB, FAR3, and MAH1, based on transcriptome and metabolome with weighted gene co-expression network analysis (WGCNA). And RT-qPCR also validated the expression patterns of these candidate genes in the glossy mutant under drought stress. This study provides new insights into the molecular mechanism of leaf epidermis wax synthesis and lays a foundation for breeding drought-resistant varieties and further functional research on the cuticular wax synthesis pathway in rapeseed.
油菜(Brassica napus L.)干旱胁迫下光泽突变体的生理、转录组和代谢组综合分析
油菜籽(Brassica napus L.)是世界上第二重要的油料作物,极易受到干旱胁迫,导致产量和质量严重下降。叶表皮蜡质在干旱胁迫中发挥着重要作用。然而,油菜叶蜡合成的遗传基础和调控机制仍不清楚。本文对干旱胁迫下的光泽突变体进行了生理学、转录组和代谢组的综合分析,以揭示油菜角质蜡积累的分子基础。转录组和代谢组的综合分析揭示了叶片中不饱和脂肪酸、蜡的生物合成和植物激素信号转导对提高耐旱性的重要作用。基于转录组和代谢组与加权基因共表达网络分析(WGCNA),确定了多个参与蜡合成途径中烷烃合成和醇合成的候选基因,包括CER1、MYB、FAR3和MAH1。RT-qPCR 也验证了这些候选基因在干旱胁迫下光泽突变体中的表达模式。该研究为叶片表皮蜡质合成的分子机制提供了新的见解,为培育抗旱品种和进一步开展油菜角质蜡质合成途径的功能研究奠定了基础。
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来源期刊
Industrial Crops and Products
Industrial Crops and Products 农林科学-农业工程
CiteScore
9.50
自引率
8.50%
发文量
1518
审稿时长
43 days
期刊介绍: Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.
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