A Zea genus-specific micropeptide controls kernel dehydration in maize

IF 45.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Pub Date : 2024-11-12 DOI:10.1016/j.cell.2024.10.030

Yanhui Yu, Wenqiang Li, Yuanfang Liu, Yanjun Liu, Qinzhi Zhang, Yidan Ouyang, Wenya Ding, Yu Xue, Yilin Zou, Junjun Yan, Anqiang Jia, Jiali Yan, Xinfei Hao, Yujie Gou, Zhaowei Zhai, Longyu Liu, Yang Zheng, Bao Zhang, Jieting Xu, Ning Yang, Jianbing Yan

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Abstract

Kernel dehydration rate (KDR) is a crucial production trait that affects mechanized harvesting and kernel quality in maize; however, the underlying mechanisms remain unclear. Here, we identified a quantitative trait locus (QTL), qKDR1, as a non-coding sequence that regulates the expression of qKDR1 REGULATED PEPTIDE GENE (RPG). RPG encodes a 31 amino acid micropeptide, microRPG1, which controls KDR by precisely modulating the expression of two genes, ZmETHYLENE-INSENSITIVE3-like 1 and 3, in the ethylene signaling pathway in the kernels after filling. microRPG1 is a Zea genus-specific micropeptide and originated de novo from a non-coding sequence. Knockouts of microRPG1 result in faster KDR in maize. By contrast, overexpression or exogenous application of the micropeptide shows the opposite effect both in maize and Arabidopsis. Our findings reveal the molecular mechanism of microRPG1 in kernel dehydration and provide an important tool for future crop breeding.

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一种玉米属特异性微肽控制玉米籽粒脱水

籽粒脱水率（KDR）是影响玉米机械化收获和籽粒质量的一个重要生产性状；然而，其潜在机制仍不清楚。在这里，我们确定了一个数量性状位点（QTL）qKDR1，它是一个非编码序列，可调控qKDR1调节肽基因（RPG）的表达。RPG 编码一种 31 个氨基酸的微肽 microRPG1，它通过精确调节乙烯信号通路中的两个基因 ZmETHYLENE-INSENSITIVE3-like 1 和 3 的表达来控制灌浆后籽粒中的 KDR。基因敲除 microRPG1 会加快玉米的 KDR。相比之下，过表达或外源应用该微肽在玉米和拟南芥中显示出相反的效果。我们的研究结果揭示了微RPG1在果仁脱水过程中的分子机制，为未来的作物育种提供了重要工具。

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来源期刊

Cell 生物-生化与分子生物学

CiteScore

110.00

自引率

0.80%

发文量

396

审稿时长

2 months

期刊介绍： Cells is an international, peer-reviewed, open access journal that focuses on cell biology, molecular biology, and biophysics. It is affiliated with several societies, including the Spanish Society for Biochemistry and Molecular Biology (SEBBM), Nordic Autophagy Society (NAS), Spanish Society of Hematology and Hemotherapy (SEHH), and Society for Regenerative Medicine (Russian Federation) (RPO). The journal publishes research findings of significant importance in various areas of experimental biology, such as cell biology, molecular biology, neuroscience, immunology, virology, microbiology, cancer, human genetics, systems biology, signaling, and disease mechanisms and therapeutics. The primary criterion for considering papers is whether the results contribute to significant conceptual advances or raise thought-provoking questions and hypotheses related to interesting and important biological inquiries. In addition to primary research articles presented in four formats, Cells also features review and opinion articles in its "leading edge" section, discussing recent research advancements and topics of interest to its wide readership.