Zhanfeng Hou, Chuan Wan, Heming Jiang, Yuena Wang, Yun Xing, Jinpeng Wang, Zhihong Liu, Xiaochun Guo, Yuhao An, Wei Han, Rui Wang, Xinhao Zhang, Feng Yin and Zigang Li
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引用次数: 0
Abstract
The application scope of various bioconjugation technologies has been expanded through the implementation of photoredox catalytic bioconjugation technology, establishing a novel biomolecular framework with exceptional residue selectivity. Herein, we report a facile acylation reaction utilizing a thioacid and a photoexcited riboflavin tetraacetate (RFTA) catalyst under visible light (450 nm). The optimal reaction conditions were obtained using a high-throughput microfluidic-based chemical robotic system and GPR model. We have successfully modified a range of proteins and antibodies and utilized the functional handle to attach diverse biological molecules, demonstrating the versatility and generality of our approach. In addition, this photoredox catalytic reaction was also successfully employed in peptide modification, protein labeling, and antibody conjugation.
期刊介绍：
ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels.
The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.
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