Aggregation-induced emission (AIE) of Au(I)-GSH complexes activated by cationic polymer for sensitive foodborne pathogens detection and inactivation

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-09 DOI:10.1016/j.foodchem.2024.141988

Ruimeng Sun, Jiayu Guo, Yurou Wang, Han Wang, Haoran Zheng, Yanfei Qi

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引用次数: 0

Abstract

Aggregation-induced emission (AIE) is a novel signal output method but is limited in pathogen sensing. Herein, a multifunctional biosensor based on the AIE properties of Au(I)-GSH complexes as signal conversion tags was firstly constructed for rapid and sensitive total bacteria. Bacteria were captured by the boronic acid group of MNPs@Au@4-MPBA (MAu@MPBA) through recognition of peptidoglycan on their surface. Simultaneously, cationic polymer Poly (diallyldimethylammonium chloride) (PDDA) were electrostatic absorb on bacteria. After magnetic separation, the remaining PDDA induced Au(I)-GSH complexes aggregation to produce strong red fluorescence, which was linearly with the quantity of bacteria. Under optimized conditions, quantitative detection of bacteria can be achieved within 60 min, with a minimum detection concentration of 18 CFU/mL. Moreover, 90 % bacteria can be effectively inactivated while being detected. This strategy is capable of sensitively detecting and killing foodborne pathogens and can be successfully applied to food safety monitoring.

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阳离子聚合物激活的 Au(I)-GSH 复合物的聚集诱导发射 (AIE)，用于灵敏检测和灭活食源性病原体

聚集诱导发射（AIE）是一种新颖的信号输出方法，但在病原体传感方面存在局限性。本文首次构建了一种基于 Au（I）-GSH 复合物的 AIE 特性的多功能生物传感器，作为信号转换标签，用于快速灵敏地检测细菌总数。通过识别细菌表面的肽聚糖，MNPs@Au@4-MPBA（MAu@MPBA）的硼酸基团捕获了细菌。同时，阳离子聚合物聚（二烯丙基二甲基氯化铵）（PDDA）静电吸附细菌。磁分离后，剩余的 PDDA 诱导 Au(I)-GSH 复合物聚集，产生强烈的红色荧光，荧光与细菌数量呈线性关系。在优化条件下，细菌的定量检测可在 60 分钟内完成，最低检测浓度为 18 CFU/mL。此外，90% 的细菌可在检测过程中被有效灭活。这种策略能够灵敏地检测和杀死食源性病原体，可成功应用于食品安全监控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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