Quantitative phase imaging endoscopy with a metalens

IF 3.5 3区 医学 Q2 CHEMISTRY, MEDICINAL
Aamod Shanker, Johannes E. Fröch, Saswata Mukherjee, Maksym Zhelyeznyakov, Steven L. Brunton, Eric J. Seibel, Arka Majumdar
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Abstract

Quantitative phase imaging (QPI) recovers the exact wavefront of light from intensity measurements. Topographical and optical density maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate quantitative phase imaging at the tip of a coherent fiber bundle using chromatic aberrations inherent in a silicon nitride hyperboloid metalens. Our method leverages spectral multiplexing to recover phase from multiple defocus planes in a single capture using a color camera. Our 0.5 mm aperture metalens shows robust quantitative phase imaging capability with a \({28}^{\circ}\) field of view and 0.\({2}{\pi}\) phase resolution ( ~ 0.\({1}{\lambda}\) in air) for experiments with an endoscopic fiber bundle. Since the spectral functionality is encoded directly in the imaging lens, the metalens acts both as a focusing element and a spectral filter. The use of a simple computational backend will enable real-time operation. Key limitations in the adoption of phase imaging methods for endoscopy such as multiple acquisition, interferometric alignment or mechanical scanning are completely mitigated in the reported metalens based QPI.

Abstract Image

使用金属丝进行定量相位成像内窥镜检查
定量相位成像(QPI)可从强度测量中恢复精确的光波面。从这些量化相移中可以提取半透明微观体的地形图和光密度图。我们利用氮化硅超球面金属膜固有的色差,演示了相干光纤束顶端的定量相位成像。我们的方法利用光谱多路复用技术,在使用彩色相机进行单次捕捉时从多个离焦平面恢复相位。我们的 0.5 毫米孔径金属膜具有强大的定量相位成像能力,视场和 0.\({2}{\pi\}) 相位分辨率(在空气中为 ~ 0.\({1}{\lambda\}) ),可用于内窥镜光纤束实验。由于光谱功能直接编码在成像透镜中,因此金属膜既是聚焦元件,又是光谱滤波器。使用简单的计算后端可实现实时操作。报告中提到的基于金属膜的 QPI 完全缓解了内窥镜相位成像方法的主要限制,如多重采集、干涉对准或机械扫描。
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来源期刊
ACS Medicinal Chemistry Letters
ACS Medicinal Chemistry Letters CHEMISTRY, MEDICINAL-
CiteScore
7.30
自引率
2.40%
发文量
328
审稿时长
1 months
期刊介绍: ACS Medicinal Chemistry Letters is interested in receiving manuscripts that discuss various aspects of medicinal chemistry. The journal will publish studies that pertain to a broad range of subject matter, including compound design and optimization, biological evaluation, drug delivery, imaging agents, and pharmacology of both small and large bioactive molecules. Specific areas include but are not limited to: Identification, synthesis, and optimization of lead biologically active molecules and drugs (small molecules and biologics) Biological characterization of new molecular entities in the context of drug discovery Computational, cheminformatics, and structural studies for the identification or SAR analysis of bioactive molecules, ligands and their targets, etc. Novel and improved methodologies, including radiation biochemistry, with broad application to medicinal chemistry Discovery technologies for biologically active molecules from both synthetic and natural (plant and other) sources Pharmacokinetic/pharmacodynamic studies that address mechanisms underlying drug disposition and response Pharmacogenetic and pharmacogenomic studies used to enhance drug design and the translation of medicinal chemistry into the clinic Mechanistic drug metabolism and regulation of metabolic enzyme gene expression Chemistry patents relevant to the medicinal chemistry field.
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