ARCaDia: single-round screening of a DNA-type targeted covalent binder possessing a latent warhead†

Abstract

A covalent binder for a target protein was obtained by a direct single-round screening of a latent-warhead-modified DNA library via affinity/reactivity-based co-selection of aptameric deoxyribonucleic acid (ARCaDia), followed by a top k-mer analysis. The optimal position of the conjugated warhead on the selected aptamer was simultaneously identified.