Highly sensitive and rapid detection of Vibrio parahaemolyticus using a dual-recognition platform based on functionalized quantum dots and aptamer

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-08 DOI:10.1007/s00604-024-06821-3

Yu Chen, Ruiwen Li, Hao Shen, Nana Li, Wenjing Gao, Haipeng Guo, Bin Feng, Shaoning Yu

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引用次数: 0

Abstract

As one of the most harmful pathogenic bacteria in shrimp aquaculture, Vibrio parahaemolyticus often causes massive mortality in shrimp. Accurate and rapid detection of V. parahaemolyticus in shrimp farming is essential for avoiding huge economic losses caused by related diseases. In this study, we designed a dual-recognition platform for efficient identification and quantification of V. parahaemolyticus. First, the target bacterium was captured with magnetic beads functionalized by aptamers (Apt-MBs), and then, the broad-spectrum fluorescent probe FcMBL@CdSe-ZnS was used to detect the bacterium based on the interactions between fragment crystallizable mannose-binding lectin (FcMBL) and pathogenic bacteria. The proposed dual-recognition strategy centered around aptamers and FcMBL@CdSe-ZnS was applied to definite quantification of V. parahaemolyticus over a wide range of 10–10⁸ CFU/mL with a limit of detection of 4 CFU/mL within 55 min. The feasibility was demonstrated by using the platform to detect V. parahaemolyticus from shrimp intestine, aquaculture water, and seawater.

Graphical Abstract

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利用基于功能化量子点和适配体的双重识别平台高灵敏、快速地检测副溶血性弧菌

副溶血性弧菌是对虾养殖中最有害的病原菌之一，经常导致对虾大量死亡。准确、快速地检测对虾养殖中的副溶血性弧菌对避免相关疾病造成的巨大经济损失至关重要。在本研究中，我们设计了一个双识别平台，用于高效识别和定量分析副溶血性弧菌。首先，使用由aptamers（Apt-MBs）功能化的磁珠捕获目标细菌，然后，基于可结晶甘露糖结合凝集素片段（FcMBL）与病原菌之间的相互作用，使用广谱荧光探针 FcMBL@CdSe-ZnS 检测细菌。所提出的以适配体和 FcMBL@CdSe-ZnS 为核心的双重识别策略被应用于副溶血弧菌的定量检测，检测范围广达 10-108 CFU/mL，55 分钟内的检测限为 4 CFU/mL。利用该平台检测虾肠、养殖用水和海水中的副溶血性弧菌证明了其可行性。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.