Effects of Porphyra yezoensis polysaccharide on antioxidant and liver health of spotted sea bass, Lateolabrax maculatus

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2024-11-07 DOI:10.1007/s10499-024-01740-9

Hao Lin, Sishun Zhou, Jianrong Ma, Lumin Kong, Zhangfan Huang, Yi Lin, Longhui Liu, Yanbo Zhao, Zhongbao Li

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Abstract

This study investigated the effects of Porphyra yezoensis polysaccharide (PP) on the antioxidant and liver health of spotted sea bass (Lateolabrax maculatus). A total of 360 spotted sea bass (10.53 ± 0.02 g) were divided into 6 groups on average, and the diets of these groups were supplemented with 0, 3, 6, 9, 12, and 15 g/kg PP (control group: K, PP1, PP2, PP3, PP4, and PP5), respectively. The experiment lasted for 52 days. The results showed that, compared with the control group, the serum glutathione (GSH) content was significantly increased and the malondialdehyde (MDA) content was significantly decreased in the PP1 group. The serum catalase (CAT) activity was significantly increased in the PP1, PP2, PP3, and PP4 groups. Additionally, the superoxide dismutase (SOD) activity was significantly increased in the PP2 and PP3 groups. In terms of liver antioxidant indexes, GSH and MDA content were not significantly different among all groups. CAT activity in the PP2 and PP3 groups was significantly higher than that in the control group, and SOD activity in the PP4 groups was significantly higher than that in the control group. The activity of amylase (AMS) and trypsin (TRS) was significantly increased in the PP1, PP2, PP3, and PP4 groups compared to the control group. Additionally, the activity of lipase (LPS) was significantly increased in the PP5 group compared to the control group. Liver injury indexes showed that serum GOT and GPT activities in the PP3 group were significantly lower than those in the control group. There was no significant difference in T-CHO and TG between the experimental group and the control group. Histological analysis showed that dietary PP improved liver cell morphology and reduced fat vacuoles. Liver transcriptome analysis of the control group and the PP4 group showed that the main change pathways were arachidonic acid metabolism, purine metabolism, and fatty acid degradation. In addition, PP regulates fibroblast growth factor 19 (FGF19), solute carrier family 3,expression of member 2 (SLC3A2), angiopoietin-like 8 (ANGPTL8), thrombospondin 1 (THBS1), tyrosinase (TYR), and kelch-like protein 19 (KEAP1) genes, making adjustments to liver health and function. In summary, PP increased the antioxidant capacity and improved the liver health of spotted sea bass.

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叶枯多糖对斑鲈抗氧化和肝脏健康的影响

本研究探讨了叶豚草多糖（PP）对斑点叉尾鲈（Lateolabrax maculatus）抗氧化和肝脏健康的影响。将 360 尾斑海鲈鱼（10.53 ± 0.02 g）平均分为 6 组，在各组日粮中分别添加 0、3、6、9、12 和 15 g/kg PP（对照组：K、PP1、PP2、PP3、PP4 和 PP5）。实验持续了 52 天。结果表明，与对照组相比，PP1 组的血清谷胱甘肽（GSH）含量显著增加，丙二醛（MDA）含量显著降低。PP1、PP2、PP3 和 PP4 组的血清过氧化氢酶（CAT）活性明显提高。此外，PP2 和 PP3 组的超氧化物歧化酶（SOD）活性也明显提高。在肝脏抗氧化指标方面，各组的 GSH 和 MDA 含量无明显差异。PP2和PP3组的CAT活性明显高于对照组，PP4组的SOD活性明显高于对照组。与对照组相比，PP1、PP2、PP3 和 PP4 组的淀粉酶（AMS）和胰蛋白酶（TRS）活性明显提高。此外，与对照组相比，PP5 组的脂肪酶（LPS）活性也明显增加。肝损伤指标显示，PP3 组的血清 GOT 和 GPT 活性明显低于对照组。实验组和对照组的 T-CHO 和 TG 没有明显差异。组织学分析表明，膳食 PP 改善了肝细胞形态，减少了脂肪空泡。对照组和 PP4 组的肝脏转录组分析表明，主要的变化途径是花生四烯酸代谢、嘌呤代谢和脂肪酸降解。此外，PP还能调节成纤维细胞生长因子19（FGF19）、溶质运载家族3成员2的表达（SLC3A2）、血管生成素样8（ANGPTL8）、凝血酶原1（THBS1）、酪氨酸酶（TYR）和类凯尔奇蛋白19（KEAP1）基因，从而调整肝脏的健康和功能。总之，聚丙烯提高了斑海鲈的抗氧化能力，改善了其肝脏健康状况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.