Au@Pt@Pd nanozymes based lateral flow immunoassay for quantitative detection of SARS-CoV-2 nucleocapsid protein in nasal swab samples

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-07 DOI:10.1007/s00604-024-06819-x

Chengcheng Li, Jinhui Lu, Chao Xiang, Enhui Zhang, Xiaofang Tian, Ling Zhang, Tingting Li, Chengyao Li

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Abstract

Three-metal-core-shell nanoparticles (Au@Pt@PdNPs) providing excellent peroxidase-like activity were applied in lateral flow immunoassay (LFIA), designated as Au@Pt@Pd-LFIA, for detecting the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). An Au@Pt@Pd-LFIA was developed for quantitatively testing of SARS-CoV-2 NP with a range 0.12–31.25 ng/mL. The limit of detection (LOD) of Au@Pt@Pd-LFIA strip was 0.06 ng/mL, which was 16-fold or eightfold more sensitive than that of the gold lateral flow immunoassay (Au-LFIA) and the gold flower flow immunoassay (AF-LFIA) strips, respectively. For detection of clinical samples from nasal swabs using test strips, Au@Pt@Pd-LFIA had 84.09% sensitivity, 100% specificity, and 92.55% accuracy. In terms of detection time, the testing of Au@Pt@Pd-LFIA strip was 16 min similar to Au-LFIA (15 min) and AF-LFIA (10 min), but much shorter than ELISA (2 h). In conclusion, Au@Pt@Pd-LFIA is a sensitive, rapid, and simple test for quantitative detection of SARS-CoV-2 NP in nasal swab samples.

Graphical Abstract

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基于 Au@Pt@Pd 纳米酶的侧流免疫分析法定量检测鼻拭子样本中的 SARS-CoV-2 核头壳蛋白

将具有优异过氧化物酶样活性的三金属核壳纳米粒子（Au@Pt@PdNPs）应用于侧流免疫分析（LFIA），并命名为Au@Pt@Pd-LFIA，用于检测严重急性呼吸系统综合征冠状病毒2（SARS-CoV-2）的核壳蛋白（NP）。所开发的 Au@Pt@Pd-LFIA 可用于定量检测 SARS-CoV-2 NP，检测范围为 0.12-31.25 ng/mL。Au@Pt@Pd-LFIA条的检出限为0.06 ng/mL，灵敏度分别是金侧流免疫分析条（Au-LFIA）和金花流免疫分析条（AF-LFIA）的16倍和8倍。在使用试纸检测鼻拭子临床样本时，Au@Pt@Pd-LFIA 的灵敏度为 84.09%，特异度为 100%，准确度为 92.55%。就检测时间而言，Au@Pt@Pd-LFIA 试条的检测时间为 16 分钟，与 Au-LFIA（15 分钟）和 AF-LFIA （10 分钟）相似，但比 ELISA（2 小时）短得多。总之，Au@Pt@Pd-LFIA是一种灵敏、快速、简便的鼻拭子样本中SARS-CoV-2 NP定量检测方法。图文摘要

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.