A highly sensitive and selective label-free impedimetric immunosensor for the detection of interleukin-6 based on AuNPs@pDA@NiCo2S4@MoS2 nanocomposite

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-07 DOI:10.1007/s00604-024-06779-2

Yisi Wang, Junying Zhang, Xinxin Lv, Ya Ding, Yaolong Wang, Yuanhua Liu, Chunyong Wu, Gongjun Yang

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Abstract

A highly sensitive and selective label-free impedimetric immunosensor based on AuNPs@pDA@NiCo₂S₄@MoS₂ nanocomposite modified on the surface of a screen-printed electrode (SPE) was designed for the detection of interleukin-6 (IL-6). The distribution of NiCo₂S₄ nanoparticles on MoS₂ nanosheets was able to prevent them from agglomerating. The polydopamine (pDA) layer was coated on the surface of NiCo₂S₄@MoS₂ nanosheets by self-polymerization, which improved the stability and biocompatibility of the nanomaterial. The excellent reduction ability of pDA promoted the synthesis of gold nanoparticles (AuNPs), which increased the amount of antibody adsorption and the conductivity of the material. Finally, the antibody (Ab) of IL-6 was immobilized on the surface of AuNPs@pDA@NiCo₂S₄@MoS₂ nanocomposite. Electrochemical impedance spectroscopy (EIS) was used to detect the change of impedance before and after the immune response between Ab and IL-6 antigen (IL-6). Under the optimal experimental conditions, the relative change in impedance and the logarithmic concentration of IL-6 showed a good linear relationship in the range 1.00 to 1.00 × 10⁶ pg/mL, with a low detection limit of 0.97 pg/mL. In addition, the proposed immunosensor performed with good reproducibility, stability, and specificity. It was successfully applied to the determination of IL-6 in patient’s serum samples of head and neck carcinoma with recoveries of 98.40% to 106.5%. To sum up, the proposed label-free impedimetric immunosensor was successfully constructed for IL-6 detection in real samples.

Graphical abstract

Abstract Image

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基于 AuNPs@pDA@NiCo2S4@MoS2 纳米复合材料的检测白细胞介素-6 的高灵敏度和高选择性无标记阻抗免疫传感器

设计了一种基于丝网印刷电极（SPE）表面修饰的 AuNPs@pDA@NiCo2S4@MoS2 纳米复合材料的高灵敏、高选择性无标记阻抗免疫传感器，用于检测白细胞介素-6（IL-6）。NiCo2S4 纳米颗粒在 MoS2 纳米片上的分布能够防止它们团聚。通过自聚合作用在 NiCo2S4@MoS2 纳米片表面包覆了聚多巴胺（pDA）层，提高了纳米材料的稳定性和生物相容性。pDA 卓越的还原能力促进了金纳米颗粒（AuNPs）的合成，从而提高了抗体的吸附量和材料的导电性。最后，IL-6 抗体（Ab）被固定在 AuNPs@pDA@NiCo2S4@MoS2 纳米复合材料表面。电化学阻抗谱（EIS）用于检测抗体与 IL-6 抗原（IL-6）发生免疫反应前后的阻抗变化。在最佳实验条件下，阻抗的相对变化与 IL-6 的对数浓度在 1.00 至 1.00 × 106 pg/mL 范围内呈良好的线性关系，检测限低至 0.97 pg/mL。此外，该免疫传感器还具有良好的重现性、稳定性和特异性。该方法被成功应用于头颈癌患者血清样本中 IL-6 的检测，回收率为 98.40% 至 106.5%。总之，所提出的无标记阻抗免疫传感器成功地用于实际样品中 IL-6 的检测。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.