Experimental investigation of fracture permeability reduction process by MICP technology with Sporosarcina pasteurii cultured by different mediums

IF 5.6 1区 工程技术 Q1 ENGINEERING, GEOLOGICAL
Lei Shi, Haiyang Qiao, Xiao Yang, Bin Zhang, Jianwei Zhang
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Abstract

This study conducted a detailed investigation on the influence of the culture medium type on the permeability reduction effect through microbial-induced calcium carbonate precipitation (MICP) technology in a single rough fracture. The differences between the Sporosarcina pasteurii cultured by two mediums were compared in terms of bacterial growth properties, permeability treatment effects, distribution characteristics of induced CaCO3, and microscopic crystal characteristics. The study revealed that the culture medium did indeed impact the permeability reduction effect when treated with MICP technology, primarily related to whether urea is added in the culture medium. Two distinct permeability reduction modes were proposed for the treatment process using Sporosarcina pasteurii cultured by different mediums through one-phase injection (mixing bacterial solution and cementing solution). The permeability decreased rapidly, and the distribution of induced CaCO3 was uneven after treatment with Sporosarcina pasteurii cultured in medium containing urea, while the permeability decreased relatively slowly and the induced CaCO3 distribution was relatively uniform when treated with Sporosarcina pasteurii cultured in medium without urea. Additionally, differences in crystal morphology were observed due to variations in seepage characteristics during the treatment process with the Sporosarcina pasteurii cultured by different mediums. Finally, some investigations were given to the treatment effect optimization for the treatment process of Sporosarcina pasteurii cultured in medium containing urea. This study gave an insight to the regulation mechanism of culture medium and treatment method for the process of permeability reduction by MICP technology in the rock fracture.

Graphical abstract

用不同培养基培养巴氏孢子菌的 MICP 技术降低断裂渗透率过程的实验研究
本研究通过微生物诱导碳酸钙沉淀(MICP)技术,详细研究了培养基类型对单一粗糙裂缝渗透性降低效果的影响。比较了两种培养基培养的巴氏芽孢杆菌在细菌生长特性、渗透处理效果、诱导碳酸钙分布特征和微观晶体特征等方面的差异。研究发现,在使用 MICP 技术处理时,培养基确实会影响渗透性降低效果,这主要与培养基中是否添加尿素有关。通过单相注入(混合菌液和固井液)使用不同培养基培养巴氏芽孢杆菌的处理过程,提出了两种不同的渗透率降低模式。使用含尿素培养基培养的巴氏芽孢杆菌处理后,渗透性迅速降低,诱导 CaCO3 分布不均匀;而使用不含尿素培养基培养的巴氏芽孢杆菌处理后,渗透性降低相对缓慢,诱导 CaCO3 分布相对均匀。此外,在使用不同培养基培养巴氏孢子虫的处理过程中,由于渗流特性的不同,也观察到了晶体形态的差异。最后,对含尿素培养基培养的巴氏孢子虫处理过程的处理效果优化进行了一些研究。该研究揭示了培养基和处理方法对岩石裂缝中 MICP 技术降渗过程的调节机制。
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来源期刊
Acta Geotechnica
Acta Geotechnica ENGINEERING, GEOLOGICAL-
CiteScore
9.90
自引率
17.50%
发文量
297
审稿时长
4 months
期刊介绍: Acta Geotechnica is an international journal devoted to the publication and dissemination of basic and applied research in geoengineering – an interdisciplinary field dealing with geomaterials such as soils and rocks. Coverage emphasizes the interplay between geomechanical models and their engineering applications. The journal presents original research papers on fundamental concepts in geomechanics and their novel applications in geoengineering based on experimental, analytical and/or numerical approaches. The main purpose of the journal is to foster understanding of the fundamental mechanisms behind the phenomena and processes in geomaterials, from kilometer-scale problems as they occur in geoscience, and down to the nano-scale, with their potential impact on geoengineering. The journal strives to report and archive progress in the field in a timely manner, presenting research papers, review articles, short notes and letters to the editors.
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