Different transcriptomic and metabolomic analysis of Saccharomyces cerevisiae BY4742 and CEN.PK2-1C strains

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2024-11-07 DOI:10.1007/s00203-024-04178-y

Meihong Zhang, Shujuan Zhao

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引用次数: 0

Abstract

To establish efficient yeast cell factories, it is necessary to understand the transcriptional and metabolic changes among different yeasts. Saccharomyces cerevisiae BY4742 and CEN.PK2-1C strains are originated from different yeast strains and are commonly used as model organisms and chassis cells in molecular biology study and synthetic biology-based natural production. Metabolomic analysis showed that the BY4742 strain produced higher levels of phenylalanine, tyrosine than CEN.PK2-1C, while CEN.PK2-1C produced high levels of indoleacetaldehyde, indolepyruvate. Transcriptomic analysis showed that the two strains showed large differences in the glycolysis pathway and pyruvate metabolism pathway. CEN.PK2-1C had greater glycolysis flux than BY4742, whereas BY4742 has greater flux in the pathway of pyruvate metabolism to produce fumarate. These findings provide a basis knowledge of the metabolomic and transcriptomic differences between BY4742 and CEN.PK2-1C strains, and also provide preliminary information for strain selection for molecular biology study and synthetic biology-based natural product production.

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对酿酒酵母 BY4742 和 CEN.PK2-1C 菌株进行不同的转录组和代谢组分析

要建立高效的酵母细胞工厂，就必须了解不同酵母之间的转录和代谢变化。毕赤酵母（Saccharomyces cerevisiae）BY4742菌株和CEN.PK2-1C菌株来自不同的酵母菌株，是分子生物学研究和基于合成生物学的天然生产中常用的模式生物和底盘细胞。代谢组分析表明，BY4742菌株产生的苯丙氨酸、酪氨酸水平高于CEN.PK2-1C，而CEN.PK2-1C产生的吲哚乙醛、吲哚丙酮酸水平较高。转录组分析表明，两株菌株在糖酵解途径和丙酮酸代谢途径上存在很大差异。与 BY4742 相比，CEN.PK2-1C 的糖酵解通量更大，而 BY4742 在丙酮酸代谢途径中产生富马酸的通量更大。这些发现为了解BY4742和CEN.PK2-1C菌株代谢组和转录组的差异提供了基础知识，也为分子生物学研究和基于合成生物学的天然产物生产的菌株选择提供了初步信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.