Synergistic integration of plant derived galactomannan and MXene to produce multifunctional nanocomposites with antibacterial and osteogenic properties

IF 4.1 2区 化学 Q2 POLYMER SCIENCE
Sinduja Malarkodi Elangovan, Riza Paul, Parthiban Shanmugam, Gopal Shankar Krishnakumar
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Abstract

This study proposes to combine plant-derived galactomannan and MXene to form an organic-inorganic hydrogel network with integrative antibacterial and osteogenic properties. Oxidized galactomannan (OxGa) was blended with N-succinyl chitosan (NSC) and varying concentrations of Mxene, owing to its high reactivity and biocompatibility. The results indicated that Mxene-loaded OxGa/NSC scaffolds exhibited biomimetic topography, adequate mechanical features and excellent physicochemical properties with strong antibacterial properties against pathogenic microbes. The cell culture experiments showed dose-dependent cytotoxic behaviour. An optimized MXene content of ≤ 4 mg/ml revealed no cytotoxicity and augmented the proliferation of osteoblast cells. Similar results were obtained in gene expression analysis where ALP, COL1A1, RUNX2, and SOX2 genes showed up-regulation. In addition, the chorioallantoic membrane (CAM) assay also illustrated augmentation of angiogenesis without any toxicity. The overall results highlight the dual performance of OxGa/NSC-MXene scaffolds to exhibit anti-infection with simultaneous osteogenic properties, which is purely determined by the concentration of MXene. Therefore, regulating MXene concentration can serve as a chemical switch in imparting adequate antibacterial functions with the ability to allow new osseous generation. Our findings on the newly designed Mxene-loaded OxGa/NSC scaffolds can be a promising biomaterial for bone-related infections with a concurrent ability to promote osteogenesis.

Abstract Image

协同整合植物提取的半乳甘露聚糖和 MXene,生产出具有抗菌和成骨特性的多功能纳米复合材料
本研究拟将植物提取的半乳甘露聚糖和 MXene 结合起来,形成一种具有抗菌和成骨综合特性的有机-无机水凝胶网络。氧化半乳甘露聚糖(OxGa)与 N-琥珀酰壳聚糖(NSC)和不同浓度的 Mxene 相混合,因为 Mxene 具有高反应活性和生物相容性。结果表明,Mxene负载的OxGa/NSC支架具有仿生物形貌、适当的机械特性和优异的理化性能,对病原微生物具有很强的抗菌性。细胞培养实验显示了剂量依赖性细胞毒性行为。优化后的 MXene 含量≤ 4 mg/ml 时没有细胞毒性,并能促进成骨细胞的增殖。基因表达分析也得出了类似的结果,ALP、COL1A1、RUNX2 和 SOX2 基因出现上调。此外,绒毛膜(CAM)试验也显示了血管生成的增强,且无任何毒性。总体结果凸显了 OxGa/NSC-MXene 支架在抗感染的同时具有成骨特性的双重性能,而这完全取决于 MXene 的浓度。因此,调节 MXene 的浓度可作为一种化学开关,在赋予其充分抗菌功能的同时,还能允许新骨生成。我们对新设计的 MXene 负载 OxGa/NSC 支架的研究结果表明,它是一种很有前景的生物材料,可用于治疗与骨相关的感染,同时还能促进骨生成。
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来源期刊
Polymer
Polymer 化学-高分子科学
CiteScore
7.90
自引率
8.70%
发文量
959
审稿时长
32 days
期刊介绍: Polymer is an interdisciplinary journal dedicated to publishing innovative and significant advances in Polymer Physics, Chemistry and Technology. We welcome submissions on polymer hybrids, nanocomposites, characterisation and self-assembly. Polymer also publishes work on the technological application of polymers in energy and optoelectronics. The main scope is covered but not limited to the following core areas: Polymer Materials Nanocomposites and hybrid nanomaterials Polymer blends, films, fibres, networks and porous materials Physical Characterization Characterisation, modelling and simulation* of molecular and materials properties in bulk, solution, and thin films Polymer Engineering Advanced multiscale processing methods Polymer Synthesis, Modification and Self-assembly Including designer polymer architectures, mechanisms and kinetics, and supramolecular polymerization Technological Applications Polymers for energy generation and storage Polymer membranes for separation technology Polymers for opto- and microelectronics.
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