Extraction and characteristic properties analyses of sodium alginate derived from the Sargassum oligocystum brown seaweed alga of the Bay of Bengal

Md. Anwar Arfien Khan , Md. Monjurul Hasan , Md. Kawsar Hossain , Dabashes Adhikery , Mahmuda Hakim , Liton Chandra Mohanta , Abu Sayeed Muhammed Sharif , Ashish Kumar Sarker
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Abstract

Sodium alginate extracted from Sargassum oligocystum brown seaweed alga is an important blue economy development process of the Bay of Bengal. In the present study, sodium alginate was extracted from the brown seaweed alga collected from the Cox’s Bazar coastal area of the Bay of Bengal. The extracted product was characterized with the Ultraviolet-visible (UV–visible) spectrophotometer, Fluorescence spectrophotometer, Fourier transform-infrared radiation (FT-IR) spectrophotometer, X-ray diffractometer (XRD), and Scanning electron microscope (SEM). The product yield was optimized with different pH levels and the maximum yield was obtained at 32.5 % at pH 10.96. The moisture, and ash content of the extracted product was measured and found at 13.25 % and 4.35 %, respectively. The viscosity of the extracted product was obtained at 60.1 mPs. The UV–visible spectrophotometry showed different absorbance peaks found at 234 nm, 240 nm, 250 nm, and 264 nm which assigned the extracted product of sodium alginate, and the maximum absorption peak lies at 276.7 nm which assigned flavonoids, and their derivatives of alginates. The fluorescence spectrophotometry demonstrated a strong emission peak at 460 nm with higher intensity (200), indicating the presence of sodium alginate. The FTIR showed the characteristic peaks that demonstrated different functional groups of sodium alginate. The XRD pattern demonstrated 0.24 crystalline indexes indicating the semi-crystalline product in nature. SEM showed clear fibrils of sodium alginate morphology indicating the absence of impurity materials.
从孟加拉湾马尾藻提取的海藻酸钠及其特性分析
从马尾藻(Sargassum oligocystum)褐海藻中提取海藻酸钠是孟加拉湾蓝色经济发展过程中的一项重要内容。本研究从孟加拉湾考克斯巴扎尔沿海地区采集的褐藻中提取了海藻酸钠。使用紫外可见分光光度计、荧光分光光度计、傅立叶变换红外分光光度计、X 射线衍射仪和扫描电子显微镜对提取的产品进行了表征。根据不同的 pH 值对产品收率进行了优化,pH 值为 10.96 时收率最高,为 32.5%。经测量,提取物的水分和灰分含量分别为 13.25 % 和 4.35 %。提取物的粘度为 60.1 mPs。紫外-可见分光光度法在 234 nm、240 nm、250 nm 和 264 nm 处发现了不同的吸光度峰,这些吸光度峰代表了海藻酸钠提取物,最大吸光度峰位于 276.7 nm 处,代表了黄酮类化合物及其海藻酸盐衍生物。荧光分光光度法显示,在 460 纳米处有一个强度较高(200)的强烈发射峰,表明存在海藻酸钠。傅立叶变换红外光谱显示了海藻酸钠不同官能团的特征峰。X 射线衍射图显示结晶指数为 0.24,表明产品性质为半结晶。扫描电子显微镜(SEM)显示了清晰的海藻酸钠纤维形态,表明其中不含杂质。
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