Jun-Woo Lee , Min-Woo Lee , Kyong-Hee Nam , Seong-Jun Chun , Hee-Mock Oh , EonSeon Jin , Hyung-Gwan Lee
{"title":"Enhancement of ketocarotenoid production via heterologous expression of orange protein from Ipomoea batatas in indigenous microalga Ettlia sp.","authors":"Jun-Woo Lee , Min-Woo Lee , Kyong-Hee Nam , Seong-Jun Chun , Hee-Mock Oh , EonSeon Jin , Hyung-Gwan Lee","doi":"10.1016/j.algal.2024.103767","DOIUrl":null,"url":null,"abstract":"<div><div>Astaxanthin, one of the most powerful natural antioxidants, is used in high-value industries such as those of cosmetics, nutraceuticals, and food products derived from microalgae. In this study, <em>Ettlia</em> sp. mutants were generated by expressing two types of heterologous orange proteins, IbOr and IbOr-R96H, in which the 96<sup>th</sup> arginine of IbOr was substituted with histidine derived from <em>Ipomoea batatas</em> to enhance astaxanthin production. The <em>Ett-IbOr-R96H</em> mutant showed a 2.4-fold increase in β-carotene content compared to the wildtype (4.59 mg g<sup>−1</sup>DCW), reaching 10.82 mg g<sup>−1</sup> under high-light conditions via two-phase cultivation. Under the stress treatment combination of high-light intensity and nitrogen deprivation, total carotenoid content increased to 17.24 mg L<sup>−1</sup> and 21.94 mg L<sup>−1</sup> in <em>Ett-IbOr</em> and <em>Ett-IbOr-R96H</em>, respectively. The astaxanthin and canthaxanthin contents in <em>Ett-IbOr-R96H</em> was 4.89 mg L<sup>−1</sup> and 0.47 mg L<sup>−1</sup>, respectively, which were 1.8- and 1.5-fold higher, respectively, than those in <em>Ett-IbOr</em>. Additionally, photosynthetic efficiency (Fv/Fm) recovered in <em>Ett-IbOr-R96H</em> under dual-stress conditions compared to the wildtype while reactive oxygen species levels decreased throughout the cultivation period. Our findings suggest that the heterologous <em>IbOr</em> expression in <em>Ettlia</em> sp. may be an effective approach for enhancing the production of ketocarotenoids and improving stress resistance for industrial applications.</div></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":"84 ","pages":"Article 103767"},"PeriodicalIF":4.6000,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424003795","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Astaxanthin, one of the most powerful natural antioxidants, is used in high-value industries such as those of cosmetics, nutraceuticals, and food products derived from microalgae. In this study, Ettlia sp. mutants were generated by expressing two types of heterologous orange proteins, IbOr and IbOr-R96H, in which the 96th arginine of IbOr was substituted with histidine derived from Ipomoea batatas to enhance astaxanthin production. The Ett-IbOr-R96H mutant showed a 2.4-fold increase in β-carotene content compared to the wildtype (4.59 mg g−1DCW), reaching 10.82 mg g−1 under high-light conditions via two-phase cultivation. Under the stress treatment combination of high-light intensity and nitrogen deprivation, total carotenoid content increased to 17.24 mg L−1 and 21.94 mg L−1 in Ett-IbOr and Ett-IbOr-R96H, respectively. The astaxanthin and canthaxanthin contents in Ett-IbOr-R96H was 4.89 mg L−1 and 0.47 mg L−1, respectively, which were 1.8- and 1.5-fold higher, respectively, than those in Ett-IbOr. Additionally, photosynthetic efficiency (Fv/Fm) recovered in Ett-IbOr-R96H under dual-stress conditions compared to the wildtype while reactive oxygen species levels decreased throughout the cultivation period. Our findings suggest that the heterologous IbOr expression in Ettlia sp. may be an effective approach for enhancing the production of ketocarotenoids and improving stress resistance for industrial applications.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment