Comparative analysis of Piezo-ICSI and conventional ICSI in bovine embryo development

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2024-10-31 DOI:10.1016/j.theriogenology.2024.10.029

Fernanda Fuentes , Luis Aguila , Felipe Pérez , Erwin Muñoz , Maria Elena Arias , Ricardo Felmer

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Abstract

Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique (ART) mainly used to overcome severe male factor infertility problems in humans and animals. However, in cattle, one of the most demanded species for its meat and milk, the efficiency of this technique is low. The present study compared the effect of the piezoelectric and conventional injection systems on the preimplantational development and quality of bovine embryos generated by ICSI. Evaluations of the conditions for performing the Piezo-ICSI procedure showed that the application of a strong pulse (I4S7) was more effective in damaging the sperm plasma and acrosomal membranes prior to injection, compared to a soft pulse (I2S2, P < 0.05). In addition, Piezo-ICSI embryos without the application of exogenous activators achieved similar levels of development as Piezo-ICSI embryos activated with ionomycin and anisomycin (P > 0.05). When comparing conventional and piezoelectric injection systems, no significant differences in embryo development were observed (P > 0.05). However, embryos generated by Piezo-ICSI showed a higher embryo quality in terms of total cell number (P < 0.05). In addition, Piezo-ICSI embryos showed an expression profile of genes essential for embryonic development similar to IVF embryos (P > 0.05), in contrast to conventional ICSI-derived embryos, which presented overexpression of CASP3 and IFNT2 (P < 0.05). In conclusion, we confirmed that Piezo-ICSI is a more convenient approach than traditional ICSI, since does not require exogenous activation and generate embryos of better quality, regarding the total number of blastomeres and the pattern of gene expression observed.

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牛胚胎发育过程中的 Piezo-ICSI 与传统 ICSI 比较分析

卵胞浆内单精子注射（ICSI）是一种辅助生殖技术（ART），主要用于克服人类和动物严重的雄性因素不育问题。然而，对于牛这一肉类和奶类需求量最大的物种，该技术的效率却很低。本研究比较了压电注射系统和传统注射系统对卵胞浆内单精子显微注射法产生的牛胚胎植入前发育和质量的影响。对压电卵胞浆内单精子显微注射（Piezo-ICSI）操作条件的评估显示，与软脉冲（I2S2，P <0.05）相比，强脉冲（I4S7）在注射前对精子浆膜和顶体膜的破坏更有效。此外，未使用外源激活剂的压电-ICSI 胚胎与使用离子霉素和异霉素激活的压电-ICSI 胚胎的发育水平相似（P >；0.05）。在比较传统和压电注射系统时，未观察到胚胎发育方面的显著差异（P > 0.05）。不过，从总细胞数来看，压电卵胞浆内单精子显微注射法产生的胚胎质量更高（P <0.05）。此外，Piezo-ICSI 胚胎显示出与 IVF 胚胎相似的胚胎发育必需基因表达谱（P >；0.05），而传统 ICSI 胚胎则显示出 CASP3 和 IFNT2 的过度表达（P <；0.05）。总之，我们证实，与传统的 ICSI 相比，Piezo-ICSI 是一种更方便的方法，因为它不需要外源激活，而且从胚泡总数和观察到的基因表达模式来看，产生的胚胎质量更好。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.