Biochar and nanoscale silicon synergistically alleviate arsenic toxicity and enhance productivity in chili peppers (Capsicum annuum L.)

Abstract

Arsenic (As) contamination in agricultural soils threatens crop productivity and food safety. In this study, we examined the efficacy of biochar (BC) and silicon nanoparticles (SiNPs) as environmentally sustainable soil amendments to alleviate As toxicity in chili (Capsicum annuum L.) plants. Our findings revealed that As stress severely inhibited the growth parameters of Capsicum annuum L., and subsequently reduced yield. However, the application of BC and SiNPs into the contaminated soil significantly reversed these negative effects, promoting plant length and biomass, particularly when applied together in a synergistic manner. Arsenic stress led to increased oxidative damage, as evidenced by a 29% increase in leaf malondialdehyde content as compared to the healthy plants. Nevertheless, the synergistic (BC + SiNPs) application effectively modulated antioxidant enzyme activity, resulting in a remarkable 55% and 66% enhancement in the superoxide dismutase and catalase levels, respectively, boosting chili's resistance against oxidative stress. Similarly, BC + SiNPs amendments improved photosynthesis by 52%, stomatal conductance by 39%, soluble sugars by 42%, and proteins by 30% as compared with those of control treatment. Additionally, the combined BC + SiNPs application significantly reduced root As content by 61% and straw As by 37% as compared with the control one. Transmission electron microscopy confirmed that the synergistic use of BC and SiNPs preserved chili leaf ultrastructure, shielding against As-induced damage. Overall, the supplementation of contaminated soil with BC and SiNPs was proved to be a sustainable strategy for mitigating As toxicity in chili peppers, enhancing plant growth, physiology, and yield, and thereby food safety.