Comparison of different processed products of Allium tuberosum Rottler for the treatment of mice asthenozoospermia.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2024-10-31 Epub Date: 2024-10-28 DOI:10.21037/tau-24-274
Wenhui Wu, Xiaohong Guo, Jie Li, Min Yang, Yongai Xiong
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引用次数: 0

Abstract

Background: Allium tuberosum Rottler improves sexual function and is used in the treatment of impotence and spermatorrhea. However, its chemical composition and mechanism of action remain unclear. This study investigates the chemical composition and mechanism of action of Allium tuberosum Rottler co-processed with salt and wine (GZP) in modulating testicular mitochondrial autophagy for the treatment of asthenozoospermia in mice.

Methods: Adenine gavage + cyclophosphamide intraperitoneal injection was used to establish the model of asthenozoospermia, and six Allium tuberosum Rottler processed products were compared in the pharmacological efficacy for the treatment of asthenozoospermia in mice. The liquid chromatograph mass spectrometer (LC-MS) assay was performed to analyse the compositional changes in the GZP. The mechanism of GZP in the treatment of asthenozoospermia in mice was further investigated. The mitophagy was detected by transmission electron microscope (TEM) and immunofluorescence, respectively. Reactive oxygen species (ROS) were detected by probe. Protein expression was determined by Western blotting.

Results: GZP exhibited optimal therapeutic effects on asthenozoospermia in mice. It showed the best therapeutic effect in improving the total number of spermatozoa, sperm survival rate, improving sperm viability and reducing sperm deformity rate, alleviating the abnormal pathological morphology of mice testis, and increasing the serum testosterone (T), follicle-stimulating hormone (FSH) and prolactin (PRL) levels in mice. The LC-MS detection found that Allicin showed the most significant increase in GZP. Besides, GZP reduced ROS level and inhibited mitophagy in mice testicular tissues. Meanwhile, it restrained the expression of PINK1, Parkin, Light chain 3II (LC3-II)/Light chain 3I (LC3-I) and Caspase-3 proteins.

Conclusions: GZP improves asthenozoospermia via inhibiting excessive mitophagy and protects the integrity of mitochondria by blocking the PINK1/Parkin signaling pathway. During which, the Allicin may play an important role.

比较用于治疗小鼠无精子症的不同块茎薤(Allium tuberosum Rottler)加工产品。
背景:块茎薤白(Allium tuberosum Rottler)可改善性功能,用于治疗阳痿和遗精。然而,其化学成分和作用机制仍不清楚。本研究探讨了与盐和葡萄酒(GZP)共同加工的块茎薤白(Allium tuberosum Rottler)在调节睾丸线粒体自噬以治疗小鼠无精症方面的化学成分和作用机制:方法:采用腺嘌呤灌胃+环磷酰胺腹腔注射的方法建立小鼠无精子症模型,比较6种薤白加工品治疗小鼠无精子症的药理作用。采用液相色谱质谱仪(LC-MS)分析了 GZP 的成分变化。进一步研究了 GZP 治疗小鼠无精子症的机制。透射电子显微镜(TEM)和免疫荧光分别检测了有丝分裂。用探针检测活性氧(ROS)。蛋白表达通过 Western 印迹检测:结果:GZP 对小鼠无精子症具有最佳治疗效果。在提高小鼠精子总数、精子存活率、精子活力、降低精子畸形率、缓解小鼠睾丸异常病理形态、提高小鼠血清睾酮(T)、卵泡刺激素(FSH)和催乳素(PRL)水平等方面的治疗效果最佳。LC-MS 检测发现,大蒜素对 GZP 的增加最为显著。此外,GZP 还能降低小鼠睾丸组织中的 ROS 水平,抑制有丝分裂。同时,它还抑制了 PINK1、Parkin、轻链 3II (LC3-II)/轻链 3I (LC3-I)和 Caspase-3 蛋白的表达:GZP通过抑制过度的有丝分裂来改善无精子症,并通过阻断PINK1/Parkin信号通路来保护线粒体的完整性。其中,大蒜素可能发挥了重要作用。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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