Effects of water deficit on two cultivars of Hibiscus mutabilis: A comprehensive study on morphological, physiological, and metabolic responses

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-04 DOI:10.1016/j.plaphy.2024.109269

Lu Zhang , Qian Xu , Xue Yong , Mengxi Wu , Beibei Jiang , Yin Jia , Jiao Ma , Lisha Mou , Shengwen Tang , Yuanzhi Pan

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引用次数: 0

Abstract

Hibiscus mutabilis, commonly known as the cotton rose, is a widely cultivated ornamental and has been acclaimed as the representative flower of the 2024 World Horticultural Exposition. The growth and ornamental characteristics of Hibiscus mutabilis can be affected by drought stress. Therefore, we investigated the physiological and metabolic responses of drought-sensitive Hibiscus mutabilis JRX-1 and drought-tolerant Hibiscus mutabilis CDS-4 under drought stress. The results of the physiological analyses revealed that, compared to JRX-1,CDS-4 maintained good growth and greater water use efficiency through stronger antioxidant defences, osmoregulatory capacity and stomatal regulation. A total of 3277 metabolites were identified in positive and negative ion modes, of which 663 metabolites presented changes in expression under drought conditions, including 306 upregulated metabolites and 357 downregulated metabolites. Secondary metabolites, such as flavonoids and diterpenoids, are crucial in the plant response to drought stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the differentially aboundant metabolites were significantly enriched in the pathways valine, leucine and isoleucine degradation; linoleic acid metabolism; one carbon pool by folate; and folate biosynthesis. The results of this study will not only help to elucidate and apply the physiological and metabolic regulatory strategies of Hibiscus mutabilis to improve its adaptation to water deficit conditions, but will also provide valuable guidance to breeders and molecular biologists in the screening and use of drought resistant genes in ornamental plants.

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缺水对变异木槿两个栽培品种的影响：形态、生理和代谢反应的综合研究。

木芙蓉（Hibiscus mutabilis）俗称棉花玫瑰，是一种广泛栽培的观赏植物，被誉为 2024 年世界园艺博览会的代表花卉。干旱胁迫会影响木芙蓉的生长和观赏特性。因此，我们研究了干旱胁迫下对干旱敏感的变异木槿JRX-1和耐旱的变异木槿CDS-4的生理和代谢反应。生理分析结果表明，与JRX-1相比，CDS-4通过更强的抗氧化防御能力、渗透调节能力和气孔调节能力，保持了良好的生长和更高的水分利用效率。正负离子模式共鉴定出 3277 个代谢物，其中 663 个代谢物在干旱条件下表达发生了变化，包括 306 个上调代谢物和 357 个下调代谢物。黄酮类和二萜类等次生代谢物是植物应对干旱胁迫的关键。京都基因组百科全书》（KEGG）通路分析表明，差异丰度代谢物显著富集在缬氨酸、亮氨酸和异亮氨酸降解；亚油酸代谢；叶酸一碳池；叶酸生物合成等通路中。本研究的结果不仅有助于阐明和应用变异木槿的生理代谢调控策略，提高其对水分亏缺条件的适应性，还将为育种家和分子生物学家筛选和利用观赏植物的抗旱基因提供有价值的指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.