Multi-scale coupling between LFP and EMG in mice by low- intensity pulsed ultrasound stimulation with different number of tone-burst.

Abstract

Low-intensity pulsed ultrasound stimulation (LIPUS) as a non-invasive, high-spatial resolution and high penetration depth brain modulation technology has been used for modulating neuromuscular function. However, the modulation of neural electrical signal changes in the neuromuscular system by LIPUS remains to be explored. In this study, we stimulated the mouse brain motor cortex by LIPUS with different number of tone burst (NTB) and recorded the local field potential (LFP) signals of the target region and electromyography (EMG) of tail muscle. Multi-Scale Transfer Entropy (MSTE) analysis method was used to explore the multi-scale synchronization characteristics and functional cortico-muscular coupling (FCMC) strength changes of mice LFP-EMG before and after LIPUS under different NTBs. The results show that the MSTE of LFP-EMG before and after LIPUS stimulation was higher than that of EMG-LFP. After adding multi-scale, MSTE has a significant relationship with time scales. When NTB = 200, the scale of extremum is the largest. There was a fitting intersection between LFP-EMG and EMG-LFP scale 7-21 before and after stimulation. After scale averaging, the LFP-EMG after stimulation was lower than that before stimulation, and the EMG-LFP after stimulation was higher than that before stimulation.Conclusion: There is a significant correlation between NTB and time scale before and after LIPUS,as well as upward and downward. Consequently,This study used FCMC methods to study different NTBs and multi-scale relationships, provides new variables from LIPUS parameters and analysis, and provides new reference for clinical applications of LIPUS.