Evaluating the Impact of Rapid Multiplex PCR Testing on Meningitis and Encephalitis Diagnosis: a Retrospective Analysis.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2024-11-01 DOI:10.7754/Clin.Lab.2024.240612

Ying-Ju Chen, Tze-Kiong Er

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引用次数: 0

Abstract

Background: Meningitis and encephalitis are life-threatening neurological emergencies requiring prompt diagnosis and treatment. Traditional diagnostic methods often lack the speed and sensitivity needed for rapid clinical decision-making. This study evaluates the effectiveness of rapid multiplex PCR testing in diagnosing meningitis and encephalitis.

Methods: Between March 28, 2022, and May 31, 2024, we analyzed cerebrospinal fluid collected from a cohort of patients aged 24 to 87 at Asia University Hospital. These specimens were analyzed using the FilmArray Meningitis/Encephalitis (ME) Panel.

Results: The study included 19 patients who underwent FilmArray ME panel testing. Of these, 2 (10.5%) tested positive, while 17 (89.5%) tested negative. Among the positive specimens, herpes simplex virus-1 was detected in one patient, and Varicella zoster virus in the other.

Conclusions: This study underscores the clinical value of the FilmArray ME panel as a rapid and reliable tool for diagnosing meningitis and encephalitis. Multiplex PCR significantly improves diagnostic accuracy and reduces time to diagnosis, facilitating faster clinical intervention and improved patient outcomes.

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评估快速多重 PCR 检测对脑膜炎和脑炎诊断的影响：一项回顾性分析。

背景：脑膜炎和脑炎是危及生命的神经系统急症，需要及时诊断和治疗。传统的诊断方法往往缺乏快速临床决策所需的速度和灵敏度。本研究评估了快速多重 PCR 检测在诊断脑膜炎和脑炎方面的有效性：方法：2022 年 3 月 28 日至 2024 年 5 月 31 日期间，我们分析了从亚洲大学医院一组 24 至 87 岁患者身上采集的脑脊液。这些标本使用 FilmArray 脑膜炎/脑炎（ME）检测板进行分析：研究包括 19 名接受 FilmArray ME 面板检测的患者。其中，2 例（10.5%）检测结果呈阳性，17 例（89.5%）检测结果呈阴性。在阳性标本中，一名患者检测出单纯疱疹病毒-1，另一名患者检测出水痘带状疱疹病毒：本研究强调了 FilmArray ME 检测试剂盒作为诊断脑膜炎和脑炎的快速可靠工具的临床价值。多重 PCR 显着提高了诊断准确性，缩短了诊断时间，有助于更快地进行临床干预和改善患者预后。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.