Magnitude and timescales of Ca isotope variability in human urine: implications for bone mass balance monitoring.

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metallomics Pub Date : 2024-11-07 DOI:10.1093/mtomcs/mfae050
François L H Tissot, Dylan Cleveland, Rosa Grigoryan, Michael A Kipp, Roxana T Shafiee, Emily Miaou, Rithika Chunduri, Hayward Melton, Theo Tacail, Dan Razionale
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Abstract

Calcium (Ca) isotopes in blood/urine are emerging biomarkers of bone mineral balance (BMB) in the human body. While multiple studies have investigated Ca isotopes in patients suffering from diseases affecting BMB, comparatively little effort has been devoted to understanding the homeostasis of Ca isotopes in healthy individuals. Here, we report on a longitudinal study of the urine Ca isotope composition (δ44/42CaUrine) from 22 healthy participants (age 19-60) over timescales ranging from days to months. Data from a single participant collected over a 30-day period show that morning urine is an excellent proxy for 24-h pooled urine fractions. Data from all participants reveal large inter-individual variability in δ44/42CaUrine (up to 2.2‰), which is partly due to anthropometric differences, as shown by a correlation between the participants' body mass index (BMI) and δ44/42CaUrine values. In contrast, intra-individual data reveal encouraging stability (within ∼±0.2-0.3‰) over timescales >160 days, indicating that self-referencing approaches for BMB monitoring hold greater promise than cross-sectional ones. Our data confirm that intra-individual δ44/42CaUrine variations are mainly a function of Ca reabsorption in the kidney, but also reveal the impact of other (and at times equally important) drivers, such as diet, alcohol consumption, physical exercise, or fasting. We also find that a magnetic resonance imaging contrast agent (gadolinium) can lead to artifacts during Ca isotope analysis. Based on our results, a series of practical considerations for the use of Ca isotopes in urine as tracers of BMB are presented.

人体尿液中钙同位素变化的幅度和时间尺度:对骨量平衡监测的影响。
血液/尿液中的钙(Ca)同位素是人体骨矿物质平衡(BMB)的新兴生物标志物。虽然已有多项研究对影响骨矿物质平衡的疾病患者体内的钙同位素进行了调查,但对健康人体内钙同位素平衡的了解却相对较少。在此,我们报告了一项对 22 名健康参与者(19 至 60 岁)的尿液钙同位素组成(δ44/42CaUrine)进行的纵向研究,研究时间跨度从几天到几个月不等。一名参与者在 30 天内收集的数据显示,晨尿是 24 小时尿液组分集合的最佳代表。来自所有参与者的数据显示,δ44/42CaUrine 的个体间差异很大(最大可达 2.2‰),这部分是由于人体测量差异造成的,参与者的体重指数(BMI)与δ44/42CaUrine 值之间的相关性就表明了这一点。与此相反,个体内部数据显示,在时间跨度大于 160 天的情况下,个体内部数据具有令人鼓舞的稳定性(在 ±0.2-0.3 ‰ 范围内),这表明采用自我参照方法监测 BMB 比采用横断面方法更有前途。我们的数据证实,个体内部δ44/42CaUrine的变化主要是肾脏对钙的重吸收作用,但也揭示了其他(有时同样重要)驱动因素的影响,如饮食、饮酒、体育锻炼或禁食。我们还发现,磁共振成像造影剂(钆)会导致钙同位素分析过程中出现伪影。基于我们的研究结果,我们提出了将尿液中的钙同位素用作 BMB 示踪剂的一系列实际考虑因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Metallomics
Metallomics 生物-生化与分子生物学
CiteScore
7.00
自引率
5.90%
发文量
87
审稿时长
1 months
期刊介绍: Global approaches to metals in the biosciences
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