Self-absorbing multilayer skin-like composite with Phyllostachys nigra polysaccharides promotes wound healing

IF 23.2 2区 材料科学 Q1 MATERIALS SCIENCE, COMPOSITES
Kui Zhao, Luming Qi, Qi Li, Yin Wang, Cheng Qian, Zhengjun Shi
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引用次数: 0

Abstract

Biocompatible and mechanically stable porous hydrogels are promising materials for artificial skin in wound healing. The rigid PVA phase was selected to make the scaffold, and a dense and uniform porous phase was formed in both transverse and longitudinal phases by freezing casting technology. The names PVA and PVA + PNS2A were used in in vitro activity tests using polymer solutions that had not been molded before. The crosslinked materials were named Base, CP1 (0.1% PNS2A), and CP2 (0.5% PNS2A). HUVEC and HaCat cell proliferation experiments proved to be safe and non-toxic and significantly promoted proliferation activity after 48 h. The cell model with Raw 264.7 proved anti-inflammatory effect by mixed polymer solution. The scratch test showed that the CP2 promotes the migration of HaCat cells more effectively at 48 h. The cell adhesion experiment showed that the skin-like composite material grew rapidly along the void direction. Animal experiments showed that the multi-layer skin-like CP2 increased the mechanical properties, gelation, and hemostasis (58.8% reduction) and inhibits inflammation through active polysaccharide in the early stage of wound healing. It promoted collagen formation in the late stages of the healing process. Upon contact with the wound, the material undergoes gelation to isolate the wound and completely healed the hurt after 12 days. Stent disintegration and 48-h absorption time provide support for implant material development.

含有黑百合多糖的自吸收多层类肤复合材料可促进伤口愈合
具有生物相容性和机械稳定性的多孔水凝胶是伤口愈合过程中很有前景的人造皮肤材料。我们选择了硬质 PVA 相来制作支架,并通过冷冻铸造技术在横向和纵向形成致密均匀的多孔相。在体外活性测试中,使用了未经模塑的聚合物溶液,命名为 PVA 和 PVA + PNS2A。交联材料分别命名为 Base、CP1(0.1% PNS2A)和 CP2(0.5% PNS2A)。HUVEC 和 HaCat 细胞增殖实验证明,混合聚合物溶液安全无毒,48 小时后可显著促进细胞增殖活性。划痕实验表明,CP2 在 48 小时后能更有效地促进 HaCat 细胞的迁移。动物实验表明,在伤口愈合早期,多层类肤质 CP2 通过活性多糖增加了伤口的机械性能、凝胶化和止血效果(减少 58.8%),并抑制了炎症。在愈合过程的后期,它能促进胶原蛋白的形成。与伤口接触后,材料发生凝胶化,隔离伤口,12 天后伤处完全愈合。支架崩解和 48 小时的吸收时间为植入材料的开发提供了支持。
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来源期刊
CiteScore
26.00
自引率
21.40%
发文量
185
期刊介绍: Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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