Modulation of soy flour bioactivity against enterotoxigenic Escherichia coli by fermentation with exopolysaccharides-producing lactic acid bacteria

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2024-10-28 DOI:10.1016/j.carbpol.2024.122922

Theodorus Eko Pramudito , Cynthia Klostermann , Eddy J. Smid , Henk A. Schols

{"title":"Modulation of soy flour bioactivity against enterotoxigenic Escherichia coli by fermentation with exopolysaccharides-producing lactic acid bacteria","authors":"Theodorus Eko Pramudito , Cynthia Klostermann , Eddy J. Smid , Henk A. Schols","doi":"10.1016/j.carbpol.2024.122922","DOIUrl":null,"url":null,"abstract":"<div><div>Enterotoxigenic Escherichia coli (ETEC)-mediated diarrhea can be mitigated by inhibiting bacterial adhesion to intestinal surface. Some lactic acid bacteria (LAB) produce exopolysaccharides (EPS) that can inhibit ETEC adhesion. In this study, we fermented soy flour-based dough (SoyD) with EPS-producing LAB strains Pediococcus pentosaceus TL (PpTL), Leuconostoc citreum TR (LcTR), Leuconostoc mesenteroides WA (LmWA) and L. mesenteroides WN (LmWN) to improve anti-adhesive activity of the dough against ETEC. The strains LcTR, LmWA and LmWN produced EPS in SoyD fermentation with similar polysaccharide yields and compositions as when grown in liquid medium, whereas PpTL was unable to produce EPS in SoyD. LcTR produced high molecular weight (Mw) dextran (∼900 kDa) while LmWA and LmWN produced dextran and levan with diverse Mw (∼20–1000 kDa). SoyD fermentation by EPS-producing LAB increased the capability of the SoyD extracts to adhere to ETEC cells and block ETEC adhesion to porcine mucin. After Mw-based fractionation, all extract-fractions (>3 kDA) of LmWA- and LmWN-fermented SoyD retained their blocking activity indicating that various Mw populations of the EPS contributes to bioactivity against ETEC. This study shows the potential of EPS-producing LAB strains as fermenting microorganisms in the development of a functional food product with anti-diarrheal properties.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"348 ","pages":"Article 122922"},"PeriodicalIF":10.7000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724011482","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Enterotoxigenic Escherichia coli (ETEC)-mediated diarrhea can be mitigated by inhibiting bacterial adhesion to intestinal surface. Some lactic acid bacteria (LAB) produce exopolysaccharides (EPS) that can inhibit ETEC adhesion. In this study, we fermented soy flour-based dough (SoyD) with EPS-producing LAB strains Pediococcus pentosaceus TL (PpTL), Leuconostoc citreum TR (LcTR), Leuconostoc mesenteroides WA (LmWA) and L. mesenteroides WN (LmWN) to improve anti-adhesive activity of the dough against ETEC. The strains LcTR, LmWA and LmWN produced EPS in SoyD fermentation with similar polysaccharide yields and compositions as when grown in liquid medium, whereas PpTL was unable to produce EPS in SoyD. LcTR produced high molecular weight (Mw) dextran (∼900 kDa) while LmWA and LmWN produced dextran and levan with diverse Mw (∼20–1000 kDa). SoyD fermentation by EPS-producing LAB increased the capability of the SoyD extracts to adhere to ETEC cells and block ETEC adhesion to porcine mucin. After Mw-based fractionation, all extract-fractions (>3 kDA) of LmWA- and LmWN-fermented SoyD retained their blocking activity indicating that various Mw populations of the EPS contributes to bioactivity against ETEC. This study shows the potential of EPS-producing LAB strains as fermenting microorganisms in the development of a functional food product with anti-diarrheal properties.

Abstract Image

查看原文本刊更多论文

用产生外多糖的乳酸菌发酵调节大豆面粉对肠毒性大肠埃希氏菌的生物活性

肠毒性大肠杆菌（ETEC）引起的腹泻可通过抑制细菌粘附到肠道表面来缓解。一些乳酸菌（LAB）产生的外多糖（EPS）可以抑制 ETEC 的粘附。在本研究中，我们用产生 EPS 的 LAB 菌株 Pediococcus pentosaceus TL (PpTL)、Leuconostoc citreum TR (LcTR)、Leuconostoc mesenteroides WA (LmWA) 和 L. mesenteroides WN (LmWN) 发酵大豆面粉面团（SoyD），以提高面团对 ETEC 的抗粘附活性。LcTR、LmWA和LmWN菌株在SoyD发酵过程中产生了EPS，其多糖产量和组成与在液体培养基中生长时相似，而PpTL则无法在SoyD中产生EPS。LcTR 产生高分子量（Mw）葡聚糖（∼900 kDa），而 LmWA 和 LmWN 则产生不同分子量（∼20-1000 kDa）的葡聚糖和利凡。产生 EPS 的 LAB 发酵 SoyD 可提高 SoyD 提取物粘附 ETEC 细胞和阻断 ETEC 粘附猪粘蛋白的能力。经过基于 Mw 的分馏后，LmWA 和 LmWN 发酵的 SoyD 的所有提取物馏分（>3 kDA）都保留了其阻断活性，这表明 EPS 的不同 Mw 群体对 ETEC 具有生物活性。这项研究表明，产生 EPS 的 LAB 菌株作为发酵微生物在开发具有止泻特性的功能性食品方面具有潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.