Genome-wide identification and expression analysis of autophagy-related genes (ATGs), revealing ATG8a and ATG18b participating in drought stress in Phoebe bournei

IF 4.5 2区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental and Experimental Botany Pub Date : 2024-10-31 DOI:10.1016/j.envexpbot.2024.106012

Hui Liu , Yan Liu , Yuting Zhang , Xiao Han , Qi Yang , Shijiang Cao , Zaikang Tong , Junhong Zhang

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引用次数: 0

Abstract

As a highly conserved intracellular degradation process in eukaryotes, autophagy plays an important role in plant response to abiotic stresses such as drought. The aim of this study was to identify the autophagy-related genes (ATGs) in Phoebe bournei, and investigate the role of PbATG8a and PbATG18b in improving drought tolerance. In this study, a total of 35 ATGs were identified in P. bournei, and the basic physical and chemical properties, phylogenetic relationship, chromosomal location, gene structure, conserved domain and cis-interacting elements in promoters of the PbATGs were analyzed. The expression patterns of PbATGs showed most PbATGs were response to PEG simulated drought treatment and ABA treatment, of which PbATG8a and PbATG18b were further selected for further research. PbATG8a and PbATG18b were transformed into yeast to improve drought tolerance, respectively. Overexpression of the number of autophagosomes. The overexpression of PbATG8a and PbATG18b in P. bournei increased the number of autophagosomes and the expression levels of other ATGs, such as PbATG5, PbATG7 and PbATG12, which may be involved in the response to drought stress. Overall, we identified the ATGs and investigated the roles of PbATG8a and PbATG18b under drought stress, which provided a research basis and reference for the study of PbATGs in P. bournei.

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自噬相关基因（ATGs）的全基因组鉴定和表达分析，揭示 ATG8a 和 ATG18b 在伯尔尼蛙干旱胁迫中的参与作用

自噬是真核生物中高度保守的细胞内降解过程，在植物应对干旱等非生物胁迫的过程中发挥着重要作用。本研究旨在鉴定波氏囊藻的自噬相关基因（ATGs），并研究 PbATG8a 和 PbATG18b 在提高耐旱性方面的作用。本研究共鉴定了35个伯尔尼蛙噬菌体ATGs，分析了PbATGs的基本理化性质、系统发育关系、染色体位置、基因结构、保守结构域和启动子中的顺式相互作用元件。PbATGs的表达模式表明，大多数PbATGs对PEG模拟干旱处理和ABA处理有响应，其中PbATG8a和PbATG18b被选作进一步研究的对象。将 PbATG8a 和 PbATG18b 分别转化到酵母中以提高其耐旱性。自噬体数量的过表达。在布尔尼酵母中过表达 PbATG8a 和 PbATG18b 增加了自噬体的数量和其他 ATGs（如 PbATG5、PbATG7 和 PbATG12）的表达水平，这些 ATGs 可能参与了对干旱胁迫的响应。总之，我们鉴定了干旱胁迫下的ATGs，并研究了PbATG8a和PbATG18b在干旱胁迫下的作用，为研究布氏菌中的PbATGs提供了研究基础和参考。

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来源期刊

Environmental and Experimental Botany 环境科学-环境科学

CiteScore

9.30

自引率

5.30%

发文量

342

审稿时长

26 days

期刊介绍： Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.