Menglong Liu, Yiyao Liu, Jingyi Xiao, Yi Ren, Xue Gao
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引用次数: 0
Abstract
Ofloxacin (OFL) is an effective antibiotic against bacteria. However, the overuse of ofloxacin can lead to residues in animal-derived foods, posing a potential threat to human health. Due to the issue of false positives in single-mode detection, it is crucial to establish a multi-mode method for accurate and sensitive detection of ofloxacin. Peroxidase-like active iron-nickel bimetallic organic framework (FeNi-MOF) and orange fluorescent carbon dots (OCDs) synthesized by a simple one-pot method were used to establish a dual-mode colorimetric/ratiometric fluorescence biosensor for the detection of ofloxacin residues. In colorimetric mode, in the presence of hydrogen peroxide (H2O2), FeNi-MOF can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce blue oxTMB. However, the fluorescence of OCDs at 588 nm will be quenched by oxTMB, thus forming a ratiometric fluorescence signal with the fluorescence of FeNi-MOF at 420 nm. The linear range of the dual-mode detection method was 10−7-10−2 mg/mL, and the detection limits were 1.1 ng/kg (colorimetric) and 0.34 ng/kg (radiometric fluorescence). In addition, the constructed smartphone sensing platform is also well applied for on-site portable detection. Therefore, we have established a dual-mode sensor with high specificity, accuracy, and excellent anti-interference ability for OFL detection, and satisfactory results are also obtained in the actual spiked samples.
期刊介绍:
Food Control is an international journal that provides essential information for those involved in food safety and process control.
Food Control covers the below areas that relate to food process control or to food safety of human foods:
• Microbial food safety and antimicrobial systems
• Mycotoxins
• Hazard analysis, HACCP and food safety objectives
• Risk assessment, including microbial and chemical hazards
• Quality assurance
• Good manufacturing practices
• Food process systems design and control
• Food Packaging technology and materials in contact with foods
• Rapid methods of analysis and detection, including sensor technology
• Codes of practice, legislation and international harmonization
• Consumer issues
• Education, training and research needs.
The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.