A BDD-Au/SPCE-based electrochemical DNA aptasensor for selective and sensitive detection of theophylline

IF 4.3 3区材料科学 Q2 MATERIALS SCIENCE, COATINGS & FILMS

Diamond and Related Materials Pub Date : 2024-10-30 DOI:10.1016/j.diamond.2024.111725

Yeni Wahyuni Hartati , Ratu Shifa Syafira , Irkham Irkham , Salma Nur Zakiyyah , Jarnuzi Gunlazuardi , Takeshi Kondo , Qonita Kurnia Anjani , Prastika Krisma Jiwanti

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Abstract

The proposed aptasensor using a DNA aptamer for the detection of theophylline (THEO) is being reported for the first time, as previous methods predominantly employed RNA aptamers. DNA aptamers have attracted significant attention due to their advantages over antibodies, including greater stability and cost-effectiveness, making them ideal for various diagnostic applications as biosensors. THEO, a drug commonly used in the treatment of respiratory diseases such as asthma, requires precise monitoring in the bloodstream due to its narrow therapeutic index. In this paper, we present the first optimization of THEO detection using a DNA aptamer-based sensor (aptasensor), utilizing a composite boron-doped diamond/Gold (BDD-Au) on screen-printed carbon electrodes. Thiolated DNA aptamers were immobilized on the BDD-Au surface, where they selectively targeted THEO in biological samples. Optimization of the aptasensor was achieved using a Box-Behnken design, determining optimal conditions of 3.0 μM for aptamer concentration, 90 min for aptamer immobilization time, and 1.0 mM for tris(2-carboxyethyl)phosphine concentration. The resulting DNA aptasensor demonstrated excellent selectivity for THEO, with a linear detection range from 1 to 1.0 mM and a detection limit of 52 nM. The sensor maintained stability for up to four weeks and exhibited high recovery rates when tested in blood serum, highlighting its potential for clinical monitoring of THEO levels.

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基于 BDD-Au/SPCE 的选择性灵敏检测茶碱的电化学 DNA 合传感器

由于以前的方法主要使用 RNA 合体，而此次提出的使用 DNA 合体检测茶碱（THEO）的合体传感器是首次报道。与抗体相比，DNA适配体具有更高的稳定性和成本效益等优点，是各种诊断应用的理想生物传感器，因此备受关注。THEO 是一种常用于治疗哮喘等呼吸系统疾病的药物，由于其治疗指数较窄，因此需要对血液进行精确监测。在本文中，我们利用丝网印刷碳电极上的掺硼金刚石/金（BDD-Au）复合材料，首次优化了基于 DNA 合体的传感器（aptasensor）对 THEO 的检测。硫醇化 DNA 合体被固定在 BDD-Au 表面上，并选择性地靶向生物样品中的 THEO。采用 Box-Behnken 设计方法对适配体传感器进行了优化，确定了最佳条件：适配体浓度为 3.0 μM，适配体固定时间为 90 分钟，三（2-羧乙基）膦浓度为 1.0 mM。由此产生的 DNA 类似物传感器对 THEO 具有极佳的选择性，线性检测范围为 1 至 1.0 mM，检测限为 52 nM。该传感器可保持稳定长达四周，在血清中进行检测时也表现出很高的回收率，这凸显了它在临床监测 THEO 水平方面的潜力。

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来源期刊

Diamond and Related Materials 工程技术-材料科学：综合

CiteScore

6.00

自引率

14.60%

发文量

702

审稿时长

2.1 months

期刊介绍： DRM is a leading international journal that publishes new fundamental and applied research on all forms of diamond, the integration of diamond with other advanced materials and development of technologies exploiting diamond. The synthesis, characterization and processing of single crystal diamond, polycrystalline films, nanodiamond powders and heterostructures with other advanced materials are encouraged topics for technical and review articles. In addition to diamond, the journal publishes manuscripts on the synthesis, characterization and application of other related materials including diamond-like carbons, carbon nanotubes, graphene, and boron and carbon nitrides. Articles are sought on the chemical functionalization of diamond and related materials as well as their use in electrochemistry, energy storage and conversion, chemical and biological sensing, imaging, thermal management, photonic and quantum applications, electron emission and electronic devices. The International Conference on Diamond and Carbon Materials has evolved into the largest and most well attended forum in the field of diamond, providing a forum to showcase the latest results in the science and technology of diamond and other carbon materials such as carbon nanotubes, graphene, and diamond-like carbon. Run annually in association with Diamond and Related Materials the conference provides junior and established researchers the opportunity to exchange the latest results ranging from fundamental physical and chemical concepts to applied research focusing on the next generation carbon-based devices.