Diagnostic Implications of CD63 and CD64 Expression Levels and FcγRIIIA 158 V/F Gene Polymorphism in Primary Immune Thrombocytopenia Adult Patients

IF 2.2 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2024-11-06 DOI:10.1111/ijlh.14391

Dina Samir Elsaid, Tamer Abd Elhamid Elbedewy, Nema Ali Soliman, Kamal Ali Shalaby, Riham Abdel-Hamid Haroun

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引用次数: 0

Abstract

Objective

Immune thrombocytopenic purpura (ITP) is an acquired autoimmune disease characterized by reduced platelet counts due to immune system dysregulation caused by many factors, including genetics, autoimmune diseases, infections, and inflammations. Therefore, the current study aimed to evaluate immunological markers such as the expression level of lysosomal associated membrane protein 3 (LAMP-3), also known as CD63, and the expression level of Fc-gamma receptor I (FcγRI), also known as CD64 and also investigate the association of Fc-gamma receptor IIIA (FcγRIIIA) 158 V/F polymorphism to the risk of ITP.

Methods

A total of 180 subjects; 60 ITP patients, 60 patients with thrombocytopenia of other causes and 60 controls were enrolled into our study. The expression level of CD63 was done using reverse transcription quantitative PCR (RTqPCR), while CD64 expression level was done by flow cytometry. The polymorphism of FcγRIIIA 158 V/F gene was analyzed by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) analysis. Finally, CD63 and CD64 protein–protein interactions were done by using the STRING online database.

Results

The expression of CD63 was significantly elevated in ITP patients than thrombocytopenia patients and healthy control. Also there was high expression level of CD64 on granulocytes and monocytes from ITP patients than other groups. Receiver operating characteristic curve (ROC curve) analysis of CD63 showed an area under the curve (AUC) revealed of 1.00, sensitivity of 100% and specificity of 100%; while for CD64 on granulocytes, AUC of 0.998 as well as a sensitivity of 96.66% and specificity of 93.33%. Regarding FcγRIIIa 158 V/F polymorphism, all patients and healthy volunteers included in this study showed the wild FF genotype.

Conclusions

The expression of both CD63 and CD64 were significantly increased in ITP patients and could be good biomarkers to diagnose ITP. Additionally, there is no association between FcγRIIIa 158 V/F polymorphism and the risk of ITP disease.

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原发性免疫性血小板减少症成人患者 CD63 和 CD64 表达水平及 FcγRIIIA 158 V/F 基因多态性的诊断意义

目的：免疫性血小板减少性紫癜（ITP）是一种获得性自身免疫性疾病，其特征是由于遗传、自身免疫性疾病、感染和炎症等多种因素导致的免疫系统失调引起的血小板数量减少。因此，本研究旨在评估溶酶体相关膜蛋白 3（LAMP-3）（又称 CD63）和 Fcγ 受体 I（FcγRI）（又称 CD64）的表达水平等免疫学标志物，并调查 Fcγ 受体 IIIA（FcγRIIIA）158 V/F 多态性与 ITP 风险的相关性：研究共招募了 180 名受试者，其中包括 60 名 ITP 患者、60 名其他原因引起的血小板减少症患者和 60 名对照组受试者。采用逆转录定量 PCR（RTqPCR）检测 CD63 的表达水平，采用流式细胞术检测 CD64 的表达水平。FcγRIIIA 158 V/F 基因的多态性是通过聚合酶链反应和限制性片段长度多态性（PCR-RFLP）分析得出的。最后，利用 STRING 在线数据库对 CD63 和 CD64 蛋白-蛋白相互作用进行了分析：结果：与血小板减少症患者和健康对照组相比，ITP患者的CD63表达明显升高。此外，ITP 患者的粒细胞和单核细胞中 CD64 的表达水平也高于其他组别。对 CD63 的接收者操作特征曲线（ROC 曲线）分析显示，曲线下面积（AUC）为 1.00，敏感性为 100%，特异性为 100%；而粒细胞上的 CD64 的接收者操作特征曲线（AUC）为 0.998，敏感性为 96.66%，特异性为 93.33%。关于 FcγRIIIa 158 V/F 多态性，本研究中的所有患者和健康志愿者都显示出野生 FF 基因型：结论：ITP 患者 CD63 和 CD64 的表达均显著增加，可作为诊断 ITP 的良好生物标志物。此外，FcγRⅢa 158 V/F 多态性与 ITP 疾病风险之间没有关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.