Development and in vitro assessment of injectable, adhesive, and self-healing chitosan-based hydrogels for treatment of spinal cord injury

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2024-10-29 DOI:10.1016/j.bioadv.2024.214090

Cátia Correia , Daniela Peixoto , Diana Soares da Costa , Rui L. Reis , Iva Pashkuleva , Natália M. Alves

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Abstract

Injured spinal cords have a limited ability to regenerate because of the inhibitory environment formed in situ that affects neuronal regrow. Ensuring stable contact between the injuried nerves to support neural regeneration in the lesion microenvironment remains a significant challenge. To address this challenge, we have engineered a new injectable and adhesive hydrogel to treat spinal cord injuries. This hydrogel was produced by functionalizing chitosan with catechol groups and crosslinking it with different amounts of β-glycerophosphate to obtain adhesive hydrogels with tunable mechanical properties. The softest hydrogel (G' ~ 300 Pa) demonstrated strong adhesion to different biological soft tissues, including porcine skin (adhesion strength of 3.4 ± 0.9 kPa) and spinal cord, as well as injectability and self-healing abilities, making it ideal for a minimally invasive administration in difficult-to-reach areas. Additionally, this composition promoted the attachment, viability, proliferation, and the expression of neuronal marker β-III tubulin (Tuj-1) by the neuroblastoma SH-SY5Y cells. Moreover, SH-SY5Y cells cultured on the hydrogel modulated its mechanical properties (G' ~ 3500 Pa). In summary, we propose a material that is compatible with different therapies for soft tissue healing, including repairing injured nerve tissue.

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开发和体外评估用于治疗脊髓损伤的可注射、粘性和自愈合壳聚糖水凝胶。

由于原位形成的抑制性环境会影响神经元的再生，因此损伤脊髓的再生能力有限。确保受伤神经之间的稳定接触以支持病变微环境中的神经再生仍然是一项重大挑战。为了应对这一挑战，我们设计了一种新型可注射粘合水凝胶来治疗脊髓损伤。这种水凝胶是用儿茶酚基团对壳聚糖进行官能化，并用不同量的β-甘油磷酸酯对其进行交联，从而获得具有可调机械性能的粘性水凝胶。最软的水凝胶（G' ~ 300 Pa）显示出与不同生物软组织的强粘附性，包括猪皮肤（粘附强度为 3.4 ± 0.9 kPa）和脊髓，以及可注射性和自愈合能力，使其成为在难以到达的区域进行微创给药的理想选择。此外，这种成分还能促进神经母细胞瘤 SH-SY5Y 细胞的附着、存活、增殖和神经元标记物 β-III 管蛋白（Tuj-1）的表达。此外，在水凝胶上培养的 SH-SY5Y 细胞还能调节其机械性能（G' ~ 3500 Pa）。总之，我们提出了一种与软组织愈合（包括修复受伤的神经组织）的不同疗法兼容的材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!