Enhancement of Antioxidative and Anti-Inflammatory Activities of Corn Silk Polysaccharides After Selenium Modification.

IF 4.2 2区医学 Q2 IMMUNOLOGY

Journal of Inflammation Research Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.2147/JIR.S467665

Yu-Yun Zheng, Xin-Yi Tong, Da-Ying Zhang, Jian-Ming Ouyang

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引用次数: 0

Abstract

Objective: This study aimed to study the effect of selenium modification on the bioactivity of corn silk polysaccharides, particularly its antioxidant and anti-inflammatory functions.

Methods: HNO₃-NaSeO₃ was used to selenize degraded corn silk polysaccharides (DCSP). The structure and physicochemical properties of DCSP and selenized corn silk polysaccharides (Se-DCSP) were characterized by inductively coupled plasma emission spectroscopy, Fourier-transform infrared, ultraviolet-visible spectroscopy, nuclear magnetic resonance, nanometer, scanning electron microscopy, and thermogravimetric analysis. The protective effects of DCSP and Se-DCSP on HK-2 cells damaged by nano-calcium oxalate and the changes of inflammatory factors were detected by laser confocal microscopy, flow cytometry, and fluorescence microscopy.

Results: The selenium content of DCSP and Se-DCSP were 19.5 and 1226.7 μg/g, respectively. Compared with DCSP, Se-DCSP showed significantly improved biological activity, including the scavenging ability of various free radicals (increased by about 2-3 times), the intracellular reactive oxygen content (decreased by about 1.5 times), and the mitochondrial membrane potential (decreased by about 2.5 times). Moreover, cell viability and morphological recovery ability were improved. Compared with DCSP, Se-DCSP significantly down-regulated HK-2 cell inflammatory factors MCP-1 (about 1.7 times), NLRP3, and NO (about 1.5 times).

Conclusion: The antioxidant activity and the ability to down-regulate the expression of inflammatory factors of Se-DCSP were significantly enhanced compared with DCSP, and Se-DCSP can better protect HK-2 cells from oxidative damage, indicating that Se-DCSP has a stronger potential ability to inhibit kidney stone formation.

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硒改性后玉米丝多糖抗氧化和抗炎活性的增强

研究目的方法：采用 HNO3-NaSeO3 对降解玉米丝多糖（DCSP）进行硒化。采用电感耦合等离子体发射光谱、傅立叶变换红外光谱、紫外-可见光谱、核磁共振、纳米、扫描电子显微镜和热重分析等方法对降解玉米丝多糖（DCSP）和硒化玉米丝多糖（Se-DCSP）的结构和理化性质进行了表征。激光共聚焦显微镜、流式细胞仪和荧光显微镜检测了 DCSP 和 Se-DCSP 对受纳米草酸钙损伤的 HK-2 细胞的保护作用以及炎症因子的变化：结果：DCSP 和 Se-DCSP 的硒含量分别为 19.5 和 1226.7 μg/g。与 DCSP 相比，Se-DCSP 的生物活性明显提高，包括清除各种自由基的能力（提高约 2-3 倍）、细胞内活性氧含量（降低约 1.5 倍）和线粒体膜电位（降低约 2.5 倍）。此外，细胞活力和形态恢复能力也得到了提高。与 DCSP 相比，Se-DCSP 能显著下调 HK-2 细胞的炎症因子 MCP-1（约 1.7 倍）、NLRP3 和 NO（约 1.5 倍）：结论：与DCSP相比，Se-DCSP的抗氧化活性和下调炎症因子表达的能力明显增强，能更好地保护HK-2细胞免受氧化损伤，表明Se-DCSP具有更强的抑制肾结石形成的潜在能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation Research Immunology and Microbiology-Immunology

CiteScore

6.10

自引率

2.20%

发文量

658

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.