Juvenile hormone controls trehalose metabolism by regulating trehalase 2 activity in ovarian development of Helicoverpa armigera.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qianyue Qin, Bo Zhang, Bin Fang, Yanpeng Chang, Xiang Li, Shiheng An, Wenli Zhao
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Abstract

Trehalase (Treh) is crucial for ovarian development as it directly regulates the energy supply by hydrolyzing trehalose into glucose. Juvenile hormone (JH) is also essential for ovarian development, but how it affects Treh2 activity remains unclear. This study, which employed Helicoverpa armigera as a model, showed that HaTreh2 transcription and enzymatic activity peaks coincided with the peak of JH titers (the 2 and 3 days after emergence). Compared to the dsGFP control, knockdown of HaTreh2 transcription severely impaired ovarian development. LC-MS/MS and site mutation experiments demonstrated that JH triggered the serine 345 phosphorylation of HaTreh2 via the GPCR-cAMP-PKA pathway, thereby activating its enzymatic activity. Additionally, HaTreh2 is directly bound with trehalose transporter (HaTreT) under JH induction, thus controlling intracellular trehalose and glucose contents as well as the transcription of HaTreT. TreT controls the amount of trehalose, which serves as a substrate for Treh1, entering the cell. Treh2, on the other hand, uses extracellular trehalose as substrate, and the hydrolysis product glucose is further transported into the cell. Here, HaTreh2 regulated the substrate that HaTreh1 can act upon in the cell by directly binding with HaTreT during ovarian development when JH is induced. Therefore, JH systematically regulated trehalose metabolism during ovarian development through regulating the activity of HaTreh2. This study sheds light on the coordinated interplay between JH pathway and sugar metabolism in ovarian development.

幼年激素通过调节 Helicoverpa armigera 胚胎发育过程中曲卤糖酶 2 的活性来控制曲卤糖代谢。
曲哈糖酶(Trehalase,Treh)通过将曲哈糖水解为葡萄糖直接调节能量供应,因此对卵巢发育至关重要。幼年激素(JH)对卵巢发育也至关重要,但它如何影响 Treh2 的活性仍不清楚。本研究以 Helicoverpa armigera 为模型,结果表明 HaTreh2 的转录和酶活性峰值与 JH 滴度峰值(出苗后 2 天和 3 天)相吻合。与dsGFP对照组相比,敲除HaTreh2转录会严重影响卵巢发育。LC-MS/MS 和位点突变实验表明,JH 通过 GPCR-cAMP-PKA 途径触发 HaTreh2 的丝氨酸 345 磷酸化,从而激活其酶活性。此外,在 JH 诱导下,HaTreh2 直接与海藻糖转运体(HaTreT)结合,从而控制细胞内海藻糖和葡萄糖的含量以及 HaTreT 的转录。TreT 控制着作为 Treh1 底物的曲哈糖进入细胞的数量。另一方面,Treh2 使用细胞外的曲哈糖作为底物,水解产物葡萄糖进一步被转运到细胞内。在这里,HaTreh2 通过在卵巢发育过程中诱导 JH 时直接与 HaTreT 结合来调节 HaTreh1 在细胞中的作用底物。因此,在卵巢发育过程中,JH通过调节HaTreh2的活性系统地调节了三卤糖代谢。这项研究揭示了卵巢发育过程中JH通路与糖代谢之间的协调相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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