Comprehensive Genome Analysis of Colistin-Only-Sensitive KPC-2 and NDM1-1-Coproducing Klebsiella pneumoniae ST11 and Acinetobacter baumannii ST2 From a Critically Ill Patient With COVID-19 in Saudi Arabia: Whole Genome Sequencing (WGS) of K. pneumoniae ST11 and A. baumannii ST2.

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-10-29 eCollection Date: 2024-01-01 DOI:10.1155/2024/9233075
Ibrahim A Al-Zahrani, Thamer M Brek
{"title":"Comprehensive Genome Analysis of Colistin-Only-Sensitive KPC-2 and NDM1-1-Coproducing <i>Klebsiella pneumoniae</i> ST11 and <i>Acinetobacter baumannii</i> ST2 From a Critically Ill Patient With COVID-19 in Saudi Arabia: Whole Genome Sequencing (WGS) of <i>K. pneumoniae</i> ST11 and <i>A. baumannii</i> ST2.","authors":"Ibrahim A Al-Zahrani, Thamer M Brek","doi":"10.1155/2024/9233075","DOIUrl":null,"url":null,"abstract":"<p><p>The COVID-19 pandemic has intensified the issue of multidrug-resistant (MDR) infections, particularly in intensive care units (ICUs). This study documents the first known case of coinfection with two extensively drug-resistant (XDR) bacterial isolates in a critically ill patient with COVID-19 in Saudi Arabia. Both XDR isolates were recovered from blood and were resistant to all tested antimicrobial agents except colistin. Whole genome sequencing (WGS) revealed that the <i>K. pneumoniae</i> isolate KP-JZ107 had sequence type 11 (ST11) and core genome MLST (cgMLST 304742), while the <i>A. baumannii</i> isolate AB-JZ67 had ST2 and cgMLST 785. KP-JZ107 was found to possess the virulence plasmid KpVP-type-1, carbapenemase genes <i>bla</i> <sub><i>NDM</i></sub> and <i>bla</i> <sub><i>KPC</i></sub> , and numerous antimicrobial-resistant genes (ARGs). The AB-JZ67 isolate had several biofilm-related genes, including biofilm-associated protein (BAP), csuE, and pgaB, and multiple ARGs, including <i>bla</i> <sub><i>ADC</i>-25</sub>, <i>bla</i> <sub><i>OXA</i>-23</sub>, and <i>bla</i> <sub><i>OXA</i>-66</sub>. Our findings suggest that the coexistence of KP-JZ107 and AB-JZ67 isolates may indicate their widespread presence in ICUs, requiring comprehensive surveillance studies across all hospitals.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":"2024 ","pages":"9233075"},"PeriodicalIF":2.8000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537734/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/9233075","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The COVID-19 pandemic has intensified the issue of multidrug-resistant (MDR) infections, particularly in intensive care units (ICUs). This study documents the first known case of coinfection with two extensively drug-resistant (XDR) bacterial isolates in a critically ill patient with COVID-19 in Saudi Arabia. Both XDR isolates were recovered from blood and were resistant to all tested antimicrobial agents except colistin. Whole genome sequencing (WGS) revealed that the K. pneumoniae isolate KP-JZ107 had sequence type 11 (ST11) and core genome MLST (cgMLST 304742), while the A. baumannii isolate AB-JZ67 had ST2 and cgMLST 785. KP-JZ107 was found to possess the virulence plasmid KpVP-type-1, carbapenemase genes bla NDM and bla KPC , and numerous antimicrobial-resistant genes (ARGs). The AB-JZ67 isolate had several biofilm-related genes, including biofilm-associated protein (BAP), csuE, and pgaB, and multiple ARGs, including bla ADC-25, bla OXA-23, and bla OXA-66. Our findings suggest that the coexistence of KP-JZ107 and AB-JZ67 isolates may indicate their widespread presence in ICUs, requiring comprehensive surveillance studies across all hospitals.

沙特阿拉伯 COVID-19 重症患者中对秋水仙碱敏感的 KPC-2 和 NDM1-1 产菌肺炎克雷伯菌 ST11 和鲍曼不动杆菌 ST2 的全基因组分析:肺炎克雷伯菌 ST11 和鲍曼不动杆菌 ST2 的全基因组测序 (WGS)。
COVID-19 大流行加剧了耐多药(MDR)感染问题,尤其是在重症监护病房(ICU)。本研究记录了已知的首例沙特阿拉伯 COVID-19 重症患者同时感染两种广泛耐药(XDR)细菌分离株的病例。这两种 XDR 分离物都是从血液中回收的,对除可乐定以外的所有测试抗菌药均有耐药性。全基因组测序(WGS)显示,肺炎克氏菌分离株 KP-JZ107 的序列类型为 11(ST11),核心基因组 MLST(cgMLST 304742),而鲍曼不动杆菌分离株 AB-JZ67 的序列类型为 ST2,核心基因组 MLST 为 785。研究发现,KP-JZ107具有毒力质粒KpVP-type-1、碳青霉烯酶基因bla NDM和bla KPC以及大量抗菌基因(ARGs)。AB-JZ67 分离物有多个生物膜相关基因,包括生物膜相关蛋白(BAP)、csuE 和 pgaB,以及多个 ARGs,包括 bla ADC-25、bla OXA-23 和 bla OXA-66。我们的研究结果表明,KP-JZ107 和 AB-JZ67 分离物的共存可能表明它们广泛存在于重症监护病房,因此需要在所有医院开展全面的监测研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信