Molecularly imprinted electrochemical sensor based on APTES-functionalized indium tin oxide electrode for the determination of sulfadiazine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-05 DOI:10.1007/s00604-024-06781-8

Samridhi Chopra, Manisha Balkhandia, Manisha Khatak, Navya Sagar, Ved Varun Agrawal

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Abstract

An electrochemical sensor was developed for the sensitive and selective detection of sulfadiazine (SDZ), based on a molecularly imprinted polymer (MIP) film formed on an indium tin oxide (ITO) electrode through a self-assembly process. The SDZ-imprinted ITO electrode (SDZ-MIP/APTES-ITO) was prepared through in situ polymerization using sulfadiazine, methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), and 2,2′-azobisisobutyronitrile (AIBN) as the template, functional monomer, cross-linker, and initiator respectively. Before polymerization, the ITO electrode was functionalized with 3-aminopropyltriethoxysilane (APTES) to promote covalent attachment of the polymer to the electrode. After polymerization, the template molecule SDZ was removed to create selective recognition sites, forming the molecularly imprinted polymer electrode (MIP/APTES-ITO), which facilitates sulfadiazine detection. The sensor’s performance was evaluated using cyclic and differential pulse voltammetry, demonstrating a linear response in the sulfadiazine concentration range 0.1 to 300 μM, with a detection limit of 0.11 μM. The MIP-based sensor exhibited good reproducibility, repeatability, selectivity, and stability in sulfadiazine detection. Its practical applicability was confirmed by the successful quantification of sulfadiazine in spiked milk samples.

Graphical abstract

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基于 APTES 功能化氧化铟锡电极的分子印迹电化学传感器用于磺胺嘧啶的测定。

通过自组装过程在铟锡氧化物（ITO）电极上形成分子印迹聚合物（MIP）薄膜，开发了一种用于灵敏、选择性检测磺胺嘧啶（SDZ）的电化学传感器。磺胺嘧啶、甲基丙烯酸（MAA）、乙二醇二甲基丙烯酸酯（EGDMA）和 2,2'-偶氮二异丁腈（AIBN）分别作为模板、功能单体、交联剂和引发剂，通过原位聚合制备了 SDZ 压印 ITO 电极（SDZ-MIP/APTES-ITO）。聚合前，先用 3-aminopropyltriethoxysilane (APTES) 对 ITO 电极进行功能化处理，以促进聚合物与电极的共价连接。聚合完成后，去除模板分子 SDZ 以创建选择性识别位点，形成分子印迹聚合物电极（MIP/APTES-ITO），从而促进磺胺嘧啶的检测。使用循环和差分脉冲伏安法对传感器的性能进行了评估，结果表明该传感器在磺胺嘧啶浓度为 0.1 至 300 μM 的范围内呈线性响应，检测限为 0.11 μM。基于 MIP 的传感器在磺胺嘧啶检测中表现出良好的重现性、重复性、选择性和稳定性。在牛奶样品中成功定量磺胺嘧啶证实了它的实用性。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.