NezhNPV, a new biocontrol agent for Nesodiprion zhejiangensis Zhou & Xiao (Hymenoptera: Diprionidae), an emerging forest pest.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-11-06 DOI:10.1002/ps.8516

Qinghua Wang, Jicheng Zhao, Enjie Li, Austin Merchant, Zhicheng Su, Quanquan Liu, Xuguo Zhou

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引用次数: 0

Abstract

Background: Nesodiprion zhejiangensis, a multivoltine sawfly, is widely distributed in south China and has caused serious damage to forests. Historically, N. zhejiangensis management has relied heavily on synthetic chemicals. To reduce the reliance on chemical control, we previously isolated a nucleopolyhedrovirus, NezhNPV, from deceased N. zhejiangensis larvae. A subsequent pathogenicity assay confirmed its high virulence in a laboratory setting.

Results: In order to comprehensively examine the hypothesis that NezhNPV is an effective new biocontrol agent for N. zhejiangensis, we carried out a field test in Beijing, China, and characterized NezhNPV morphologically by electron microscopy and genetically by genome sequencing. Our field trials showed that NezhNPV was effective in controlling N. zhejiangensis in a naturally infested Himalayan blue pine forest. The occlusion bodies of NezhNPV consist of irregular polyhedra that occlude rod-shaped enveloped virions with a single nucleocapsid per virion. The NezhNPV genome is 80 637 bp in length, and contains 90 open reading frames, including 38 core, eight lepidopteran baculovirus, 34 hymenopteran baculovirus and 10 unique baculovirus genes, representing the smallest known genome among baculoviruses. The combined results based on phylogenetic analyses, Kimura-2-parameter distances and biological characteristics indicate that NezhNPV is a novel gammabaculovirus and candidate for species status with the provisional name Gammabaculovirus nezhejiangensis. NezhNPV is highly collinear with other gammabaculoviruses and contains nonsyntenic regions with an inversion and rearrangement between orf3 and orf35.

Conclusion: The combined results from our field trials, coupled with morphological and genomic characterization clearly demonstrate the bioactivity of NezhNPV. This gammabaculovirus may be included in pest management practices against N. zhejiangensis as a novel biocontrol agent. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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NezhNPV，一种针对新出现的森林害虫 Nesodiprion zhejiangensis Zhou & Xiao（膜翅目：双翅目）的新型生物控制剂。

背景：浙金锯螨是一种多伏锯螨，广泛分布于中国南方，对森林造成了严重破坏。一直以来，浙金锯螨的防治主要依赖于合成化学品。为了减少对化学防治的依赖，我们之前从死亡的浙金锯螨幼虫中分离出了一种核多聚核酸病毒（NezhNPV）。随后的致病性实验证实了它在实验室环境中的高致病性：为了全面验证NezhNPV是一种有效的新型浙金蝇生物控制剂这一假设，我们在中国北京进行了田间试验，并通过电子显微镜对NezhNPV进行了形态学鉴定，通过基因组测序对其进行了遗传学鉴定。野外试验结果表明，NezhNPV 能有效控制喜马拉雅蓝松林中自然侵染的浙金龟子。NezhNPV 的闭锁体由不规则多面体组成，闭锁杆状包膜病毒，每个病毒体有一个核头状体。NezhNPV 基因组全长 80 637 bp，包含 90 个开放阅读框，包括 38 个核心基因、8 个鳞翅目杆状病毒基因、34 个膜翅目杆状病毒基因和 10 个独特的杆状病毒基因，是已知杆状病毒中最小的基因组。根据系统进化分析、Kimura-2-参数距离和生物学特征的综合结果表明，NezhNPV是一种新型钩端螺旋体病毒，是候选种，暂定名为钩端螺旋体病毒（Gammabaculovirus nezhejiangensis）。NezhNPV与其他gammabaculovirus高度共线，包含非同源区，在orf3和orf35之间存在倒位和重排：田间试验的综合结果以及形态学和基因组学特征清楚地证明了 NezhNPV 的生物活性。作为一种新型的生物防治剂，NezhNPV 可被用于浙江糯稻的病虫害防治。© 2024 作者。害虫管理科学》由 John Wiley & Sons Ltd 代表化学工业协会出版。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.